Evidence map›Paper›PMID 40454464›Full record

ArticleActa crystallographica. Section F, Structural biology communications2025

Preparing for successful protein crystallization experiments.

Gabrielle R Budziszewski, Vivian Stojanoff, Sarah E J Bowman

Abstract read
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Synthetic Ligands of Myeloid C-Type Lectin Receptors.Chembiochem : a European journal of chemical biology · 2026
    Review
  2. Article
  3. Introduction to the SAMPREP special issue.Acta crystallographica. Section F, Structural biology communications · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Gabrielle R BudziszewskiUniversity at Buffalo Hauptman Woodward Institute, Buffalo, NY 14203, USA.ORCID 0000-0003-0910-8504
Vivian StojanoffNational Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.ORCID 0000-0002-6650-512X
Sarah E J BowmanUniversity at Buffalo Hauptman Woodward Institute, Buffalo, NY 14203, USA.ORCID 0000-0002-7426-7328

Funding

X-ray Scattering Technology CoreP30GM133893 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI Vivian Stojanoff · 2019 to 2026
$38.6M
National HTX Center: Enabling Access to State-of-the-Art Crystallization CapabilitiesR24GM141256 · NIGMS · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI Sarah Elizabeth Johnson Bowman · 2021 to 2026
$6.3M
National Institutes of Health, National Institute of General Medical Sciences P30GM133893National Institutes of Health, National Institute of General Medical Sciences R24GM141256NIGMS NIH HHS P30 GM133893NIGMS NIH HHS R24 GM141256U.S. Department of Energy BO070U.S. Department of Energy DE-SC0012704
6 · The paper itself

Abstract

Crystal-based structural methods, including X-ray crystallography, are frequently utilized for the determination of high-resolution structures of biomolecules. All crystal-based diffraction methods first require the preparation of biomolecular crystals, and careful sample preparation for crystallization experiments can increase the frequency of success. In this article, strategies to optimize factors that can impact crystallization are presented, from which buffers and reducing agents are most favorable to which crystallization techniques could be used.

Indexed as

ProteinsBuffersCrystallizationCrystallography, X-RayBuffersProteinscrystallizationelectron diffractionstructural biologyX-ray crystallographyX-ray free-electron lasers

Identifiers

PMID40454464
PMCPMC12210191

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.