Evidence map›Paper›PMID 40454341›Full record

ArticleACS central science2025

Novel Click Coupling Chemistry to Explore Glycan Recognition.

Tianwei Jia, Akul Y Mehta, Catherine A Tilton, Ea Kristine Clarisse Tulin, Lauren E Pepi, Lukas Muerner, Stephan von Gunten, Jamie Heimburg-Molinaro, Sean R Stowell, Richard D Cummings

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS central science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Use of Microbial Microarrays to Define Antibody Specificity.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Tianwei JiaDepartment of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, National Center for Functional Glycomics, CLS 11087-3 Blackfan Circle, Boston, Massachusetts 02115, United States.
Akul Y MehtaDepartment of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, National Center for Functional Glycomics, CLS 11087-3 Blackfan Circle, Boston, Massachusetts 02115, United States.
Catherine A TiltonDepartment of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, National Center for Functional Glycomics, CLS 11087-3 Blackfan Circle, Boston, Massachusetts 02115, United States.
Ea Kristine Clarisse TulinDepartment of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, National Center for Functional Glycomics, CLS 11087-3 Blackfan Circle, Boston, Massachusetts 02115, United States.
Lauren E PepiDepartment of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, National Center for Functional Glycomics, CLS 11087-3 Blackfan Circle, Boston, Massachusetts 02115, United States.
Lukas MuernerDepartment of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, National Center for Functional Glycomics, CLS 11087-3 Blackfan Circle, Boston, Massachusetts 02115, United States.
Stephan von GuntenInstitute of Pharmacology, University of Bern, Inselspital, INO-F, Bern 3010, Switzerland.
Jamie Heimburg-MolinaroDepartment of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, National Center for Functional Glycomics, CLS 11087-3 Blackfan Circle, Boston, Massachusetts 02115, United States.
Sean R StowellJoint Program in Transfusion Medicine, Brigham and Women's Hospital, Harvard Medical School, 630E New Research Building, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, United States.
Richard D CummingsDepartment of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, National Center for Functional Glycomics, CLS 11087-3 Blackfan Circle, Boston, Massachusetts 02115, United States.ORCID https://orcid.org/0000-0002-8918-5034

Funding

Project 2 - Convergence of innate immunity and microbial communities in the regulation of anti-blood group antibody developmentP01HL171803 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI Sean R Stowell · 2024 to 2026
$10.0M
NHLBI NIH HHS P01 HL171803
6 · The paper itself

Abstract

Specific recognition of glycans by proteins is important in many biological processes and immune responses. Here we present a general approach for derivatizing free glycans with a novel linker MTZ (3-(methoxyamino)-propylamine added to a bioorthogonal-functional tetrazine tag) that exploits click chemistry to generate multiple platforms of glycan coupling. This derivatization preserves glycan integrity, is reversible and quantifiable, and incorporates a bioorthogonal tetrazine tag for click coupling. A library of ABO-(H) blood group MTZ-glycans was efficiently conjugated to avidin Luminex beads through a Biotin-PEG11-TCO (

Identifiers

PMID40454341
PMCPMC12123462

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.