ArticleACS omega2025
Molecularly Imprinted Nanoparticle-Based Assay for Tetracycline Determination at Subnanomolar Levels.
Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
6 authors.
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Abstract
Antibiotic determination at low concentrations is a critical challenge to ensure water, food, and environmental safety. This work reports the application of molecularly imprinted nanoparticles (MINs) as synthetic antibodies in a microplate assay for tetracycline (TC) determination at subnanomolar levels. MINs with hydrodynamic radii ranging from 200 to 300 nm were synthesized via solid-phase imprinting on magnetic nanoparticles using lymecycline (LMC) as an auxiliary template. MINs immobilized onto polystyrene microplates exhibited a selective binding of the conjugate between LMC and horseradish peroxidase (HRP), yielding analytical responses up to fourfold higher than those observed in blank wells. MINs saturated with LMC-HPR showed the quantitative displacement of the conjugate upon incubation with LMC or TC, yielding concentration-proportional responses from 0.001 to 100 nmol L
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Registered trials
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