ArticleACS omega2025
D7A Mutagenesis and In Silico Analysis on Haloacid Dehalogenase Gene from Pseudomonas aeruginosa ITB1 in pET/
Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors.
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Abstract
Organohalogens, including haloalkanoic acids, are organic compounds synthesized in large quantities for various purposes in the agriculture, pharmaceutics, textile, and chemical industries. Unfortunately, organohalogen waste is persistent in the environment and toxic to many organisms, including humans. In this respect, bioremediation is considered the safest and most ecofriendly technique to reduce this class of xenobiotics. Pseudomonas aeruginosa ITB1 local strain has been identified to produce haloacid dehalogenase, a hydrolytic enzyme that catalyzes the breakage of carbon-halogen covalent bonds in haloalkanoic acids. Our previous studies have successfully cloned the haloacid dehalogenase gene from this bacterium into pGEM-T Easy in E. coli TOP10, sequenced, and then subcloned into pET-30a-(+) for expression in E. coli BL21-(DE3). The gene was named
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