ArticleJournal of inflammation research2025
Single-Cell Analysis Reveals Aspirin Restores Intervertebral Disc Integrity via Ferroptosis Regulation.
Article in Journal of inflammation research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Ferroptosis and bone health: bridging the gap between mechanisms and therapy.Frontiers in immunology · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Low back pain represents a major global health issue, with intervertebral disc degeneration (IVDD) being one of its primary causes. Disc degeneration involves complex processes such as inflammation, matrix degradation, and cell death, yet the underlying mechanisms remain poorly understood. Single-cell RNA sequencing offers a powerful approach to elucidate cellular heterogeneity and dynamic changes in IVDD, providing valuable insights for early diagnosis and targeted therapeutic strategies. Methods: The Harmony algorithm was used to integrate four independent single-cell sequencing datasets. Subtype identification, differential expression analysis, enrichment analysis, and cell proportion analysis were conducted to explore functional alterations in various nucleus pulposus cell (NPC) subpopulations. Molecular docking was employed to evaluate the stability of aspirin targeting GPX4. In vitro and in vivo experiments were performed to assess the therapeutic effects of aspirin on IVDD. Results: Eight distinct NPC subtypes were identified based on cellular heterogeneity and their associated marker genes. The CDKN1A⁺aNPC subtype increased progressively with disease severity, while the matrix-supporting ABI3BP⁺mNPC and SOD3⁺mNPC subtypes significantly decreased in advanced degeneration. Concurrently, there was an increase in ECM remodeling-related LTBP1⁺mNPCs. Within the CDKN1A⁺aNPC, GPX4 was notably downregulated, suggesting the activation of ferroptosis. Molecular docking results revealed a high affinity of aspirin for GPX4. Additionally, aspirin inhibited ferroptosis and ameliorated disc structural damage. Conclusion: The increased proportion of CDKN1A⁺aNPC cells serves as an early warning feature for the progression of IVDD. Aspirin stabilizes the targeting of GPX4, thereby inhibiting ferroptosis and exerting therapeutic effects on IVDD.
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Registered trials
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