Evidence map›Paper›PMID 40453949›Full record

ArticleVeterinary world2025

Comprehensive genome analysis of

M Salem, A Zaghawa, F Housawi, Ahmed Elsify, Mohamed E Hasan, Alyaa Elrashedy, Kazem AlMohammed Salem, Nidhal Ben Amor, A Al Naeem

Abstract read
In one paragraph

Article in Veterinary world, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

M SalemDepartment of Clinical Sciences, College of Veterinary Medicine, King Faisal University, Hofuf, Kingdom of Saudi Arabia.
A ZaghawaDepartment of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Minoufiya, Egypt.
F HousawiDepartment of Clinical Sciences, College of Veterinary Medicine, King Faisal University, Hofuf, Kingdom of Saudi Arabia.
Ahmed ElsifyDepartment of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Minoufiya, Egypt.
Mohamed E HasanDepartment of Bioinformatics, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
Alyaa ElrashedyDepartment of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Minoufiya, Egypt.
Kazem AlMohammed SalemAnimal Resources Management, Ministry of Environment, Water and Agriculture, Al-Ahsa, Saudi Arabia.
Nidhal Ben AmorDepartment of Public Health, College of Veterinary Medicine, King Faisal University, Hofuf, Kingdom of Saudi Arabia.
A Al NaeemDepartment of Clinical Sciences, College of Veterinary Medicine, King Faisal University, Hofuf, Kingdom of Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Aim: Paratuberculosis, caused by Materials and Methods: A total of 345 blood samples were collected for seroprevalence analysis using enzyme-linked immunosorbent assay (ELISA), and 68 rectal scraping samples were analyzed using Ziehl-Neelsen staining and PCR for strain differentiation. Seventeen clinical cases underwent clinical, postmortem, and histopathological examinations. Whole-genome sequencing and bioinformatics analyses were performed using the Bacterial and Viral Bioinformatics Resource Center to identify genetic variations, Results: Clinical findings revealed progressive emaciation and chronic diarrhea in affected camels. Gross examination showed intestinal wall thickening and mesenteric lymph node congestion. Histopathological analysis indicated hyperactivation of crypts of Lieberkühn and mononuclear cell infiltration. PCR analysis identified a higher prevalence of the sheep (S) strain (162 bp) compared to the cattle (C) strain (310 bp). The overall seroprevalence of MAP was 8.11% (ELISA). Whole-genome sequencing identified 34 Conclusion: This study provides critical insights into the genetic diversity and AMR mechanisms of MAP strains in camels, emphasizing the need for targeted control strategies. The findings highlight potential zoonotic risks and inform future vaccine development to mitigate MAP infections in livestock.

Indexed as

antimicrobial resistancecamel healthMycobacterium avium subsp. paratuberculosisphylogeneticsSaudi Arabiawhole-genome sequencing

Identifiers

PMID40453949
PMCPMC12123282

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.