ArticleAvicenna journal of medical biotechnology
Recombinase Polymerase Amplification (RPA)-ELISA as an Isothermal Molecular POCT Method for Bacterial Respiratory Infection Diagnosis.
Article in Avicenna journal of medical biotechnology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Emerging point-of-care technologies for bacterial pathogen detection.Journal of Zhejiang University. Science. B · 2026Review
- Recombinase Polymerase Amplification (RPA) as an Isothermal Molecular-Point of Care Test (IM-POCT) for Rapid Identification of P. aeruginosa Among Patients with Respiratory Infection.Molecular biotechnology · 2026Article
- A Systematically Enhanced LAMP Chip for Rapid, Sensitive, and Contamination-Free Pathogen Detection.ACS measurement science au · 2026Article
- Rapid and simultaneous detection ofFrontiers in cellular and infection microbiology · 2026Article
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Authors and funding
6 authors.
Funding
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Abstract
Background: Acute Respiratory Infections (ARIs) are a leading cause of childhood mortality worldwide, especially in African and Southeast Asian countries. Point of Care Test (POCT) techniques provide faster diagnoses compared to conventional or real-time PCR methods. Recombinase Polymerase Amplification (RPA) offers rapid on-site detection of these infections. Coupling RPA with Enzyme-Linked Immunosorbent Assay (ELISA) (RPA-ELISA) creates a cost-effective alternative, ideal for clinical applications. This study evaluates RPA-ELISA as a rapid diagnostic tool for bacterial respiratory infections. Methods: From 11 August 2022 to 9 February 2023, respiratory samples were collected and processed using culture methods, biochemical tests, real-time PCR, and RPA assays. The RPA reactions were conducted at 39 Results: Forty-two respiratory samples, were collected in this period of which 10 samples showed no growth, and 32 tested positive. Among these positive samples, 15 isolates (35.7%) were identified as Conclusion: RPA-ELISA is significantly faster than real-time PCR and culture methods. Its ease of use makes it suitable for on-site diagnoses in resource-limited environments. Limitations include a small sample size for certain bacteria and the necessity for further validation in varied clinical contexts.
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