Evidence map›Paper›PMID 40453851›Full record

ArticleOpen veterinary journal2025

Kinematic and morphological evaluation of frozen Friesian Holstein semen stored for 33, 30, 27, and 24 years.

Ahmad Budi Purnawan, Rimayanti Rimayanti, Suherni Susilowati, Erma Safitri, Tatik Hernawati, Imam Mustofa, Zulfi Nur Amrina Rosyada, Adeyinka Oye Akintunde, Aswin Rafif Khairullah

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Article in Open veterinary journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Ahmad Budi PurnawanSingosari National Artificial Insemination Center, Malang, Indonesia.
Rimayanti RimayantiDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Suherni SusilowatiDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Erma SafitriDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Tatik HernawatiDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Imam MustofaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Zulfi Nur Amrina RosyadaDivision of Veterinary Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Adeyinka Oye AkintundeDepartment of Agriculture and Industrial Technology, Babcock University, Ilishan Remo, Nigeria.
Aswin Rafif KhairullahResearch Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Fertility is determined by sperm motility, kinematics, and morphology. Motility is the most commonly used metric worldwide, aside from movement score and semen concentration, and is one of the semen quality indicators included in the Indonesian national standard (SNI 4869.1-2017) for frozen semen quality. Aim: This study aimed to determine the effect of frozen semen storage for 33, 30, 27, and 24 years on the sperm kinematics and morphology of frozen-thawed semen in FH bulls. Methods: Samples were collected from the frozen semen of FH bulls using five straws for each storage period. Evaluation of frozen-thawed sperm motility, kinematics, and morphology was performed using a computer-assisted sperm analysis IVOS Results: Based on statistical observation, the results of the research were not significantly different ( Conclusion: FH bull frozen-thawed semen of 33, 30, 27, and 24 years did not differ in sperm concentration, motility, kinematics, and sperm morphology and fulfilled the standards for artificial insemination.

Indexed as

CryopreservationSemenSemen AnalysisSemen PreservationSpermatozoaAnimalsBiomechanical PhenomenaCattleFreezingMaleSperm MotilityTime FactorsFH bullFood securityFrozen semen storageSperm kinematicsSperm morphology

Identifiers

PMID40453851
PMCPMC12124797

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.