Influence of Osteopontin-Coated Surface on Osteoblast Behaviour Relevant to Dental Implant Integration.

Osteopontin (OPN) plays an important role in osteogenesis mechanisms and influences bone formation and resorption. OPN contains several bioactive domains, including a hydroxyapatite (HA) binding domain and an osteoinductive sequence Ser-Val-Val-Tyr-Gly-Leu-Arg (SVVYGLR). The present study coated the OPN at concentrations of 0.5, 1.0 and 1.5 µg/mL on the glass surface to examine the effects of adhesion and spreading of a murine osteoblast cell line. After cells were seeded on the modified surfaces. Cell number and spreading were evaluated using the MTT assay and phalloidin immunocytochemistry staining. The cell attachment was examined after seeding for 1 h. Although a slight increase in cell attachment was noted in the 1.5 µg/mL OPN-coated condition. There was no statistically significant difference. Cell proliferation was significantly increased in 1 and 1.5 µg/mL OPN-coated conditions at day 7. Cell spreading was markedly noted in OPN-coated conditions compared with the control at 10 min and 3 h. However, cell morphology was similar among conditions at 24 h. Therefore, OPN could be useful for material surface modification to promote osteoblast responses.