Evidence map›Paper›PMID 40453063›Full record

ArticleBlood neoplasia2024

Instructive interaction between myelodysplastic hematopoiesis and the bone marrow microenvironment at the single-cell level.

Johann-Christoph Jann, Nanni Schmitt, Alexander Streuer, Qingyu Xu, Vladimir Riabov, Eva Altrock, Nadine Weimer, Verena Nowak, Julia Obländer, Iris Palme and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Blood neoplasia, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Johann-Christoph JannDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Nanni SchmittDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Alexander StreuerDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Qingyu XuDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Vladimir RiabovDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Eva AltrockDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Nadine WeimerDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Verena NowakDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Julia ObländerDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Iris PalmeDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Melda GölDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Marie DemmerleDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Felicitas RappDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Fabian SiegelDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Laurenz SteinerDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Mahmoud GhazalDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Angelika DudaDepartment for Clinical Chemistry, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Verena HaselmannDepartment for Clinical Chemistry, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Ali DarwichDepartment of Orthopedic Surgery, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Ahmed JawharDepartment of Orthopedic Surgery, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Mohamad JawharDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Georgia MetzgerothDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Wolf-Karsten HofmannDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Daniel NowakDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Myelodysplastic neoplasms (MDS) are hypothesized to remodel their bone marrow (BM) microenvironment to reinforce conditions for their propagation. In this study, we investigated interactions between MDS cells and the BM niche at single-cell level. In a patient-derived xenograft (PDX) model, we analyzed 13 000 cells from different murine niche cell populations after long-term (>24 weeks) exposure to MDS vs healthy human grafts. Subsequently, we analyzed over 24 000 primary human BM cells enriched for the nonhematopoietic compartment by using whole bone fragments from n = 8 patients with MDS and n = 7 healthy, age-matched donors. In PDX who received MDS transplantation, mesenchymal cell (MSC) subpopulations were forced to overexpress hematopoietic factors such as Cxcl12 and Il7 upon contact with hematopoietic MDS cells as compared with healthy grafts. Single-cell analyses of primary in situ BM cells from patients with MDS showed highly heterogeneous MSC subpopulations on a patient-individual level. We identified inflammatory gene expression profiles as well as overexpression of C-X-C Motif Chemokine Ligand 12, KIT ligand, and Interleukin 7 in MDS MSCs and endothelial cells. In conclusion, we demonstrate reprogramming of the BM microenvironment by MDS cells, pointing to altered MSC subpopulations with increased growth factor expression profiles in a subgroup of patients with MDS.

Identifiers

PMID40453063
PMCPMC12082128

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.