ArticlePeerJ2025
Gene expression profiling of extraocular muscles in primary inferior oblique overaction.
Article in PeerJ, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
3 authors.
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Abstract
Background: This study investigates gene expression differences in primary inferior oblique overaction (IOOA) by performing transcriptome sequencing on extraocular muscles (EOMs) from patients with primary and secondary IOOA. Strabismus, particularly IOOA, is often associated with abnormal eye movement due to imbalanced muscle function. By using bioinformatic analyses to identify differentially expressed genes (DEGs) and enriched pathways, we aim to uncover the molecular distinctions that may underlie the unique neuromuscular characteristics of primary IOOA. Methods: Transcriptome sequencing was conducted on EOMs from ten patients with primary IOOA and ten patients with secondary IOOA. DEGs were identified using DESeq2. Gene Ontology (GO) annotations were enriched using ClusterProfiler and GlueGo, and the overall gene expression data were analyzed with Gene Set Enrichment Analysis (GSEA). The protein-protein interaction (PPI) network of DEGs was constructed using STRING. Results: We identified 258 DEGs, with 110 genes significantly upregulated and 148 genes downregulated in the primary IOOA group compared to the secondary IOOA group. Analysis of DEGs revealed that upregulated genes in the primary group were associated with myelination ( Conclusion: Our preliminary findings suggested that neuromuscular DEGs in primary IOOA were enriched in pathways related to myelination, ion channels, M-bands, and collagen fibril. The results indicated that multiply innervated muscle fibers were more abundant in the primary IOOA group, likely enabling slow tension generation, whereas the secondary IOOA group exhibited higher collagen fibril levels that might improve muscle stiffness.
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