ArticleBMC plant biology2025
Genome-wide identification of NHX gene family and effects of salicylic acid in regulating antioxidant activity as well as nutrients deposition under salt stress in three Brassica species.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Role of primary protectors of plant cells in salinity tolerance: molecular mechanisms and adaptive strategies.Plant signaling & behavior · 2026Review
- Identification and characterization of salt-, drought-, and combined salt-drought stress-responsivePlant signaling & behavior · 2026Article
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2026Article
- Rhizobacteria-Mediated Reprogramming of Phytohormone Landscapes for Mitigating Salinity Stress in Plants.International journal of molecular sciences · 2026Review
- Foliar application of chitosan-silicon nanoparticles and salicylic acid enhances salinity tolerance and yield of common bean (Phaseolus vulgaris L.) under field conditions.BMC plant biology · 2026Article
- Genome-wide identification and salt stress-induced expression analysis of the NHX gene family in peanut (Arachis hypogaea L.).BMC plant biology · 2026Article
- Integrative Bioinformatic Analysis ofGenes · 2026Article
- Genome-wide identification and gene expression analysis of AhNHXs in peanut.BMC plant biology · 2026Article
- Genotype-dependent salt tolerance mechanisms in wheat-Thinopyrum introgression lines revealed by ion transporter gene expression and seedling phenotyping.Scientific reports · 2026Article
- Genome-wide identification and expression profiling of the NHX gene family in oat (Avena sativa L.).BMC genomics · 2026Article
- Genome-Wide Identification ofInternational journal of molecular sciences · 2026Article
- Comparative physiological and transcriptome analyzes reveal the function of exogenous dopamine in improving the tolerance to salt stress inFrontiers in plant science · 2026Article
- Molecular and morphological signatures of drought and salinity stress inFrontiers in plant science · 2026Article
- Impact of Trichoderma, abscisic acid and polyamines on resistance tomato cultivars against M. incognita infection.Molecular biology reports · 2025Article
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11 authors.
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Abstract
backgroundThe Na+/H+ antiporter (NHX) gene subfamily is essential for plant adaptation to salt stress, contributing significantly to ion homeostasis. These antiporters play crucial function in various biological processes by regulating Na+ and H+ balance within plant cells. Yet their genomic features and roles of salicylic acid (SA) in regulating them, activating antioxidant defense mechanisms as well as nutrients acquisition under salt stress conditions remain largely unknown in many crop species.
resultsIn this study, we identified the NHX gene family members of three Brassicaceae species including Brassica napus, Brassica rapa and Brassica oleracea and analyzed the expression patterns of NHX genes in response to salt stress and phytohormonal application (SA). Comprehensive computational analyses, encompassing domain and motif prediction, cellular localization, cis-regulatory elements (CREs), and post-translational modification (PTM) sites revealed distinct group-specific characteristics within the NHX gene family. These characteristics included chromosomal distribution, motif composition, exon count and protein length (amino acids). qRT-PCR analysis depicted significantly induced expression of NHXs, specifically, BnNHX7, BraNHX6 and BoNHX6 (4 ~ 6 folds) using different concentrations of NaCl (100 mM and 200 mM) and SA (10 mg/L and 20 mg/L) at different periods of time. SA induced antioxidant enzymatic activity (SOD 3 ~ 4 folds, POD 2 ~ 3 folds, CAT 1 ~ 2 folds and APX 11 ~ 12 folds), upregulated antioxidant genes (SOD, POD, APX, CAT) by 3 ~ 5 folds under salt stress, and improved nutrient acquisition in all respective Brassicaceae species.
conclusionsWe have conducted the first molecular characterization of NHX genes in three Brassica species, revealing their dual role in salt stress adaptation through ion homeostasis and salicylic acid induced antioxidant regulation, thus identifying promising targets for crop improvement. CLINICAL TRIAL NUMBER: Not applicable.
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