Evidence map›Paper›PMID 40452041›Full record

ArticleBMC cancer2025

Effects of functional antioxidants on the expansion of gamma delta T-cells and their cellular cytotoxicity against bladder cancer cells.

Yueh Pan, Hung-Jen Shih, Shu-Han Chuang, Chin-Pao Chang, Chi-Hao Hsiao, Ya-Hsu Chiu, Pai-Fu Wang, Chi-Chen Lin, Ping-Hsiao Shih

Abstract read
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In one paragraph

Article in BMC cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yueh PanDivision of Urology, Department of Surgery, Changhua Christian Hospital, No.135 Nanhsiao Street, Changhua City, 50006, Taiwan.
Hung-Jen ShihDivision of Urology, Department of Surgery, Changhua Christian Hospital, No.135 Nanhsiao Street, Changhua City, 50006, Taiwan.
Shu-Han ChuangDivision of General Practice, Department of Medical Education, Changhua Christian Hospital, No.135 Nanhsiao Street, Changhua City, 50006, Taiwan.
Chin-Pao ChangDivision of Urology, Department of Surgery, Changhua Christian Hospital, No.135 Nanhsiao Street, Changhua City, 50006, Taiwan.
Chi-Hao HsiaoDepartment of Urology, School of Medicine, College of Medicine and TMU Research Center of Urology and Kidney (TMU-RCUK), Taipei Medical University, No.250, Wuxing St., Xinyi Dist, Taipei City, 110, Taiwan.
Ya-Hsu ChiuTranslational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, No. 2, Yude Rd., North Dist, Taichung City, 404327, Taiwan.
Pai-Fu Wang *Division of Urology, Department of Surgery, Changhua Christian Hospital, No.135 Nanhsiao Street, Changhua City, 50006, Taiwan.
Chi-Chen Lin *Doctoral Program in Translational Medicine, National Chung Hsing University, No.145 Xingda Rd., South Dist, Taichung City, 402202, Taiwan.
Ping-Hsiao Shih *Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, No. 2, Yude Rd., North Dist, Taichung City, 404327, Taiwan. phs1027@gmail.com.

Funding

Changhua Christian Hospital, Taiwan 112-CCH-IRP-087
6 · The paper itself

Abstract

purposeResults of previous studies have demonstrated that T-cell receptor cross-linking rapidly generates reactive oxygen species, which play essential signaling roles within mitochondria for the antigen-specific expansion of T-cells. However, oxidative stress also causes damage to cellular organelles. Thus, modulating ROS metabolism using antioxidants during naïve T-cell activation may promote the expansion and generation of functional T-cells. Notably, urothelial cancer is a sex-specific malignancy with high mortality rates worldwide. The present study aimed to evaluate the effects of various antioxidants on γδ T-cell proliferation, and the associated cytotoxicity against urothelial carcinoma cells (UCs).

methodsOver a period of cell induction and expansion, peripheral blood mononuclear cells were cultured with or without different antioxidants, including N-acetyl cysteine (NAC), vitamin C and vitamin E. Subsequently, phenotypic characterization of γδ T-cells and their cytolytic effects against UCs were analyzed by flow cytometry and cell viability assays, respectively. RESULTS AND

conclusionsThe results revealed that NAC partially inhibited T-cell expansion in a dose-dependent manner. In addition, CD3

Indexed as

AntioxidantsCytotoxicity, ImmunologicIntraepithelial LymphocytesReceptors, Antigen, T-Cell, gamma-deltaT-LymphocytesUrinary Bladder NeoplasmsAcetylcysteineAscorbic AcidCell Line, TumorCell ProliferationCell SurvivalHumansLymphocyte ActivationOxidative StressReactive Oxygen SpeciesVitamin EAcetylcysteineAntioxidantsAscorbic AcidReactive Oxygen SpeciesReceptors, Antigen, T-Cell, gamma-deltaVitamin EAdoptive T-cell therapyCytolysisMulti-functional antioxidantOxidative stressUrothelial carcinoma of the bladderΓδ T-cell

Identifiers

PMID40452041
PMCPMC12128524

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.