ArticleNature communications2025
Charting unknown metabolic reactions by mass spectrometry-resolved stable-isotope tracing metabolomics.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Biosynthesis-Informed Putative Annotation Reveals Alkaloid Profiles Across Tissues ofMetabolites · 2026Article
- Stable Isotope-Resolved Metabolomics in Elucidating Mitochondrial Metabolic Reprogramming and Therapeutic Targets.Biomedical chromatography : BMC · 2026Review
- Leveraging 13C-Labeling to Assign Molecular Formulas to Unknown Yeast Metabolites.Journal of the American Society for Mass Spectrometry · 2026Article
- Mapping the mammalian dark metabolome bybioRxiv : the preprint server for biology · 2026Article
- Metabolic networks in the tumor microenvironment: roles of amino acid and lipid metabolism pathways in cancer progression and therapy.Experimental & molecular medicine · 2026Review
- Reduced glutathione levels inmSystems · 2026Article
- Large-Scale Metabolite Annotation in Untargeted Metabolomics Using MetDNA.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Urinary metabolome dynamics inMetabolomics : Official journal of the Metabolomic Society · 2025Article
- Review
- Mechanisms and Applications of Gastrointestinal Microbiota-Metabolite Interactions in Ruminants: A Review.Microorganisms · 2025Review
- Arbuscular Mycorrhizal Fungi as Core Engineers in Synthetic Microbial Communities: Boosting Plant Growth and Soil Health for Sustainable Agriculture.Journal of fungi (Basel, Switzerland) · 2025Review
- Advanced Characterization of Environmental Pollutant Metabolism in Human Skin.Journal of xenobiotics · 2025Article
- Charting unknown metabolic reactions by mass spectrometry-resolved stable-isotope tracing metabolomics.Nature communications · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
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Abstract
Metabolic reactions play important roles in organisms such as providing energy, transmitting signals, and synthesizing biomacromolecules. Charting unknown metabolic reactions in cells is hindered by limited technologies, restricting the holistic understanding of cellular metabolism. Using mass spectrometry-resolved stable-isotope tracing metabolomics, we develop an isotopologue similarity networking strategy, namely IsoNet, to effectively deduce previously unknown metabolic reactions. The strategy uncovers ~300 previously unknown metabolic reactions in living cells and mice. Specifically, we elaborately chart the metabolic reaction network related to glutathione, unveiling three previously unreported reactions nestled within glutathione metabolism. Among these, a transsulfuration reaction, synthesizing γ-glutamyl-seryl-glycine directly from glutathione, underscores the role of glutathione as a sulfur donor. Functional metabolomics studies systematically characterize biochemical effects of previously unknown reactions in glutathione metabolism, showcasing their diverse functions in regulating cellular metabolism. Overall, these newly uncovered metabolic reactions fill gaps in the metabolic network maps, facilitating exploration of uncharted territories in cellular biochemistry.
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