Evidence map›Paper›PMID 40449520›Full record

ArticleBioTechniques2025

Mito-Kaede photoactivation and chase experiment for mitophagy: mitophagy flux response toward various stimulations.

Yoh Sugawara, Hiroyuki Morinaga, Jingyuan Chen, Yoshinori Kitagawa, Hiroki Ogata, Asiya Karim, Miu Kikuchi, Maryam Khan, Erica Yasuhara, Takahisa Goto and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioTechniques, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yoh SugawaraDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children Boston, and Harvard Medical School, Boston, MS, USA.
Hiroyuki MorinagaDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children Boston, and Harvard Medical School, Boston, MS, USA.
Jingyuan ChenDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children Boston, and Harvard Medical School, Boston, MS, USA.
Yoshinori KitagawaDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children Boston, and Harvard Medical School, Boston, MS, USA.
Hiroki OgataDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children Boston, and Harvard Medical School, Boston, MS, USA.
Asiya KarimDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children Boston, and Harvard Medical School, Boston, MS, USA.
Miu KikuchiDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children Boston, and Harvard Medical School, Boston, MS, USA.
Maryam KhanDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children Boston, and Harvard Medical School, Boston, MS, USA.
Erica YasuharaDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children Boston, and Harvard Medical School, Boston, MS, USA.
Takahisa GotoDepartment of Anesthesiology and Critical Care Medicine, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
Joseph A Jeevendra MartynDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children Boston, and Harvard Medical School, Boston, MS, USA.
Shingo YasuharaDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children Boston, and Harvard Medical School, Boston, MS, USA.

Funding

Pilot & Feasibility ProgramP30DK043351 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI RAYMOND T CHUNG · 1991 to 2026
$35.3M
P&F programP30DK135043 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI Aaron S Leong · 2023 to 2026
$5.4M
Major Burn Injury and its Effects on Acute and Superimposed Surgical PainR01GM142042 · NIGMS · MASSACHUSETTS GENERAL HOSPITAL · PI MARTYN, JEEVENDRA · 2020 to 2023
$1.3M
NIDDK NIH HHS P30 DK043351NIDDK NIH HHS P30 DK135043NIGMS NIH HHS R01 GM142042
6 · The paper itself

Abstract

Mitophagy, a crucial mitochondrial quality control system for cellular stress adaptation, is a key focus in pathophysiology and drug discovery. Developing a simple and versatile mitophagy flux assay is vital for advancing our understanding of cellular responses. Addressing a gap in systematic methods, we employ the photoactivatable fluorescent protein mito-Kaede in C2C12 myocytes, demonstrating its remarkable versatility in quantifying mitophagy flux responses under various stimuli, including carbonyl cyanide m-chlorophenyl hydrazone (CCCP), TNF-α, lipopolysaccharide (LPS), and hypoxia. This study underscores the validity and distinctive advantages of the mito-Kaede assay through comparative analysis with conventional assays including Western blotting (WB), potentially providing valuable insights for both mitophagy flux analysis and drug development.

Indexed as

Luminescent ProteinsMitochondriaMitophagyAnimalsCarbonyl Cyanide m-Chlorophenyl HydrazoneCell LineLipopolysaccharidesMiceTumor Necrosis Factor-alphaCarbonyl Cyanide m-Chlorophenyl HydrazoneLipopolysaccharidesLuminescent ProteinsTumor Necrosis Factor-alphaAutophagyflux measurementmitochondriamito-Kaedemitophagyquantitative assaystimulated response kineticstime-lapse

Identifiers

PMID40449520
PMCPMC12584847

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.