Evidence map›Paper›PMID 40448780›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

MicroCT Scanning and Analysis of the Zebrafish Skeleton.

Arianna Ericka Gómez, Phil Salmon, Nell Baumgarten, Jyoti Rai, Philippe Huber, Susana P Simmonds, Rohda A Yase, Kiana A Reynolds, W Joyce Tang, Ronald Young Kwon

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Loss ofbioRxiv : the preprint server for biology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Arianna Ericka GómezDepartment of Orthopaedics and Sports Medicine, University of Washington, Seattle, WA, USA.
Phil SalmonBruker Belgium, Kontich, Belgium. phil.salmon@bruker.com.
Nell BaumgartenDepartment of Orthopaedics and Sports Medicine, University of Washington, Seattle, WA, USA.
Jyoti RaiDepartment of Orthopaedics and Sports Medicine, University of Washington, Seattle, WA, USA.
Philippe HuberDepartment of Orthopaedics and Sports Medicine, University of Washington, Seattle, WA, USA.
Susana P SimmondsDepartment of Orthopaedics and Sports Medicine, University of Washington, Seattle, WA, USA.
Rohda A YaseDepartment of Orthopaedics and Sports Medicine, University of Washington, Seattle, WA, USA.
Kiana A ReynoldsDepartment of Orthopaedics and Sports Medicine, University of Washington, Seattle, WA, USA.
W Joyce TangDepartment of Orthopaedics and Sports Medicine, University of Washington, Seattle, WA, USA.
Ronald Young KwonDepartment of Orthopaedics and Sports Medicine, University of Washington, Seattle, WA, USA. ronkwon@uw.edu.

Funding

Diversity Supplement for Rohda YaseR01AR074417 · NIAMS · UNIVERSITY OF WASHINGTON · PI KWON, RONALD Y · 2020 to 2025
$2.9M
NIAMS NIH HHS R01 AR074417
6 · The paper itself

Abstract

Zebrafish and other small laboratory fish have emerged as important model systems for bone biomedical research. Micro-computed tomography (microCT) is a powerful approach to non-destructively visualize and quantify bone morphology, microarchitecture, and mineral density. While consensus methods for microCT scanning analysis have been established in other vertebrates including small rodents, the small size of zebrafish, their different anatomy, and their amenability to rapid-throughput and high-content approaches call for unique considerations. In this chapter, we describe key considerations for performing microCT scans in the zebrafish skeleton. We also describe different approaches for sample mounting, scan acquisition, and analysis that can be used depending on experimental objectives and outcomes. Finally, we describe a step-by-step protocol for microCT scanning and analysis of adult zebrafish using FishCuT, resulting in hundreds of descriptors of vertebral morphology and mineral density.

Indexed as

Bone and BonesX-Ray MicrotomographyZebrafishAnimalsBone DensityImage Processing, Computer-AssistedSpineBone mineral densityFishCuTMicroarchitecturemicroCTZebrafish

Identifiers

PMID40448780

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.