Evidence map›Paper›PMID 40448759›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

2D and 3D In Vitro Co-culture for Cancer and Bone Cell Interaction Studies.

Ryan T Bishop, Jesus Delgado-Calle, Michaela R Reagan, Silvia Marino

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ryan T BishopDepartment of Tumor Microenvironment and Metastasis, H. Lee Moffitt Cancer Research Center and Institute, Tampa, FL, USA.
Jesus Delgado-CalleDepartment of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Michaela R ReaganCenter for Molecular Medicine, Maine Medical Centre Research Institute, Scarborough, ME, USA.
Silvia MarinoDepartment of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA. SMarino@uams.edu.

Funding

Defining the Roles of Bone Marrow Adipocytes and FABP4/5 Signaling in Multiple Myeloma Drug Resistance - Diversity SupplementR37CA245330 · NCI · MAINEHEALTH · PI REAGAN, MICHAELA R. · 2020 to 2025
$2.8M
Bone-Targeted Therapies to Improve Bone Health and Prevent Relapse in Multiple MyelomaR37CA251763 · NCI · UNIV OF ARKANSAS FOR MED SCIS · PI DELGADO-CALLE, JESUS · 2020 to 2025
$2.6M
NCI NIH HHS R37 CA245330NCI NIH HHS R37 CA251763
6 · The paper itself

Abstract

Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most used cell culture techniques, functional assays, and gene expression.

Indexed as

Bone and BonesCell CommunicationNeoplasmsAdipocytesAnimalsBone Marrow CellsCell Culture TechniquesCell Line, TumorCoculture TechniquesHumansOsteoblastsOsteoclastsOsteocytesAdipocytesBone marrow stromal cellsCancer cellsIn vitro co-cultureOsteoblastsOsteoclastsOsteocytes

Identifiers

PMID40448759

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.