Evidence map›Paper›PMID 40448757›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Isolation and Characterization of Primary Bone Marrow Adipocytes in Rodent Models.

Samantha N Costa, Carolyn Chlebek, Clifford J Rosen

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Samantha N CostaCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, ME, USA.
Carolyn ChlebekCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, ME, USA.
Clifford J RosenCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, ME, USA. Clifford.Rosen@mainehealth.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter will present innovative methods for the isolation and characterization of mature, primary bone marrow adipocytes from rodents. The key advantage of this protocol is that it allows for ex vivo analyses of mature bone marrow adipocytes, which is a more direct approach than traditional in vitro studies. While the difficulty in isolating mature adipocytes from the heterogenous bone marrow population has precluded their characterization in many preclinical studies, we have optimized our methods to maximize the collection of alive, mature bone marrow adipocytes and minimize contamination of other bone marrow and immune cells.

Indexed as

AdipocytesBone Marrow CellsCell SeparationAnimalsCell DifferentiationCells, CulturedMiceRatsBMAT isolationBMAT RNA isolationBMAT secretomeBone marrow adipocytes

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.