Evidence map›Paper›PMID 40448756›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Isolation, Differentiation, and Characterization of Human Bone Marrow Stromal Stem Cells In Vitro and In Vivo.

Janos Kanczler, Rahul S Tare, Howard C Tribe, Patrick Stumpf, Andrew Rawlings, Karen Marshall, Richard O C Oreffo

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Janos KanczlerBone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Human Development & Health, Institute of Developmental Sciences, University of Southampton Medical School, Southampton, UK. J.Kanczler@soton.ac.uk.
Rahul S TareBone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Human Development & Health, Institute of Developmental Sciences, University of Southampton Medical School, Southampton, UK.
Howard C TribeBone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Human Development & Health, Institute of Developmental Sciences, University of Southampton Medical School, Southampton, UK.
Patrick StumpfBone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Human Development & Health, Institute of Developmental Sciences, University of Southampton Medical School, Southampton, UK.
Andrew RawlingsBone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Human Development & Health, Institute of Developmental Sciences, University of Southampton Medical School, Southampton, UK.
Karen MarshallBone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Human Development & Health, Institute of Developmental Sciences, University of Southampton Medical School, Southampton, UK.
Richard O C OreffoBone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Human Development & Health, Institute of Developmental Sciences, University of Southampton Medical School, Southampton, UK. Richard.Oreffo@soton.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this chapter, we describe techniques for the isolation, enrichment, and characterization of skeletal stem cells from human bone marrow. The methods for enriching STRO-1 and STRO-4 positive cells using magnetic activated cell sorting are detailed, as are the techniques for establishing and characterising osteogenic, adipogenic, and chondrogenic cultures derived from these cells. Finally, we present methods for studying the ability of these enriched skeletal stem and progenitor populations to produce bone in vivo using diffusion chambers following subcutaneous implantation, over 4-6 weeks.

Indexed as

Bone Marrow CellsCell DifferentiationCell SeparationMesenchymal Stem CellsAdipogenesisAnimalsCell Culture TechniquesCells, CulturedChondrogenesisFlow CytometryHumansMiceOsteogenesisAdipocyteBone marrowChondrocyteDiffusion chamberOsteoblastStem cells

Identifiers

PMID40448756

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.