Evidence map›Paper›PMID 40448754›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Isolation and Generation of Osteoclasts.

Neha Sharma, Marija K Simic, Bethan K Davies, Jacob Bastholm Olesen, Kent Søe, Michelle M McDonald

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Neha SharmaClinical Cell Biology, Pathology Research Unit, University of Southern Denmark, Odense, Denmark.
Marija K SimicDepartment of Pathology, New York University Grossman School of Medicine, New York, NY, USA.
Bethan K DaviesDepartment of Clinical and Experimental Endocrinology, KU Leuven, Leuven, Belgium.
Jacob Bastholm OlesenDepartment of Pathology, Odense University Hospital, Odense, Denmark.
Kent SøeClinical Cell Biology, Pathology Research Unit, University of Southern Denmark, Odense, Denmark.
Michelle M McDonaldFaculty of Medicine and Health, The University of Sydney, Camperdown, NSW, Australia. michelle.mcdonald@sydney.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter describes the isolation, expansion, staining, and imaging of osteoclasts from murine (MKS, BKD, and MMM) and human (NS, JBO, and KS) sources. We cover in detail both traditional and more modern methods of assessing osteoclast formation and function in vitro including advances in image acquisition and automated analyses. Importantly, we provide in-depth methods for human osteoclast culture systems, methods to assess human osteoclast function, and highlight potential methodological pitfalls and ways to overcome them. This collection of protocols provides a valuable resource for labs either initiating in vitro osteoclast assays or aiming to expand on traditional methods.

Indexed as

Cell Culture TechniquesCell SeparationOsteoclastsAnimalsCell DifferentiationCells, CulturedHumansMiceBone resorptionCell fusionOsteoclast

Identifiers

PMID40448754

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.