Evidence map›Paper›PMID 40448747›Full record

ArticleMolecular biology reports2025

Selection of stable internal control genes for quantitative real-time PCR (RT-qPCR) in banana (Musa spp.) under short-term drought stress.

Mary Krizelle Veronica A Paragas, Elaine P Laurio, Jen Daine L Nocum, Marc Lawrence E Francisco, Darlon V Lantican, Jose Arnel O Reyes, Anna Mariel U Toledo, Anand Noel C Manohar

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Mary Krizelle Veronica A Paragas *Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, 4031, College, Laguna, Philippines.
Elaine P Laurio *Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, 4031, College, Laguna, Philippines.
Jen Daine L Nocum *Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, 4031, College, Laguna, Philippines.
Marc Lawrence E Francisco *Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, 4031, College, Laguna, Philippines.
Darlon V Lantican *Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, 4031, College, Laguna, Philippines.
Jose Arnel O Reyes *Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, 4031, College, Laguna, Philippines.
Anna Mariel U Toledo *Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, 4031, College, Laguna, Philippines.
Anand Noel C Manohar *Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, 4031, College, Laguna, Philippines. acmanohar@up.edu.ph.

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6 · The paper itself

Abstract

backgroundBanana (Musa spp.) production in the Philippines is hampered by drought, underscoring the need for drought-tolerant cultivars. RT-qPCR (quantitative real-time polymerase chain reaction) is a powerful tool for analyzing gene expression but requires stable internal control genes for accurate normalization. However, there is limited data regarding reference gene stability in local banana cultivars under drought stress. Identifying a reliable internal control gene is essential for accurate differential gene expression analysis, which can support breeding efforts for drought-tolerant cultivars. This study aims to select the most stably expressed internal control gene among five candidates (25S, ACT1, EF1-α, L2, and RPS2) in drought-stressed 'Lakatan' (AAA) and 'Saba' (ABB/BBB) cultivars. METHODS AND

resultsUnder controlled greenhouse conditions, biological triplicates per cultivar were subjected to well-watered and drought-imposed treatments. After drought imposition, total RNA was extracted from the leaf tissues, followed by complementary DNA (cDNA) synthesis. Primer efficiency (E) per candidate gene was evaluated for optimization. Quantification cycle (C

conclusionsThis study identifies L2, encoding the ribosomal protein L2, as the most stable internal control gene in drought-stressed bananas, a crucial prerequisite for differential gene expression analysis in drought-related studies. These findings allow for more accurate measurement of gene expression, thereby reinforcing the role of potential drought response genes that can be used for breeding climate-smart banana cultivars through marker-assisted selection and new breeding techniques.

Indexed as

Genes, PlantMusaReal-Time Polymerase Chain ReactionStress, PhysiologicalDroughtsGene Expression ProfilingGene Expression Regulation, PlantPlant ProteinsReference StandardsPlant ProteinsBananaDroughtGene expressionInternal control genesReference genesRT-qPCR

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PMID40448747

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