Evidence map›Paper›PMID 40448745›Full record

ArticleArchives of microbiology2025

Impacts of penicillin G on Chlamydia trachomatis serovar D and host HeLa cells in genital infections.

Mei Wang, Xiufeng Qiu, Youhong Xu, Leran Zhao, Quanzhong Liu, Cong You

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Complete deletion of theInfection and immunity · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Mei WangDepartment of Dermatology, School of Medicine, Tianjin First Central Hospital, Nankai University, Tianjin, 300190, China.
Xiufeng QiuDepartment of Dermatology and Venereology, The First Affiliated Hospital of Gannan Medical University, No. 23 Qingnian Road, Zhanggong District, Ganzhou, 341000, China.
Youhong XuCollege of Pharmacy, Gannan Medical University, Ganzhou, 341000, China.
Leran ZhaoDepartment of Dermatology and Venereology, The General Hospital of Tianjin Medical University, Tianjin, 300052, China.
Quanzhong LiuDepartment of Dermatology and Venereology, The General Hospital of Tianjin Medical University, Tianjin, 300052, China.
Cong YouDepartment of Dermatology and Venereology, The First Affiliated Hospital of Gannan Medical University, No. 23 Qingnian Road, Zhanggong District, Ganzhou, 341000, China. youcong1987@tmu.edu.cn.

Funding

Science and Technology Project of Jiangxi Health and Family Planning Committee 202510462Science and Technology Research Project of Jiangxi Education Department GJJ2401311Young Scientists Fund of the Natural Science Foundation of Jiangxi Province 20242BAB20427
6 · The paper itself

Abstract

This study investigated the impacts of penicillin G on the Chlamydia trachomatis (CT) Serovar D and host cells in urogenital infections. HeLa cells were infected with CT for 2 h and then treated with different concentrations of penicillin G. The aberrant CT group was compared to untreated CT using dual RNA sequencing, lncRNA sequencing, and bioinformatics analysis. Treatment with 150 U/ml penicillin G for 48 h caused significant abnormalities in CT inclusion bodies. A total of 93 differentially expressed mRNAs were involved in biological processes such as protein modification, cofactor biosynthesis, and response to stimuli. Key pathways affected included peptidoglycan biosynthesis and degradation, oxidative phosphorylation, and bacterial secretion systems. Additionally, penicillin G induced significant changes in CT morphology and mRNA expression, as well as in host HeLa cellular mRNA and lncRNA profiles. These changes may contribute to chronic inflammation, treatment resistance, and potentially tumorigenesis.

Indexed as

Anti-Bacterial AgentsChlamydia InfectionsChlamydia trachomatisPenicillin GReproductive Tract InfectionsFemaleHeLa CellsHumansRNA, Long NoncodingRNA, MessengerSerogroupAnti-Bacterial AgentsPenicillin GRNA, Long NoncodingRNA, MessengerChlamydia trachomatisLong non-coding RNAmRNAPenicillin

Identifiers

PMID40448745

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.