ArticleReproductive sciences (Thousand Oaks, Calif.)2025
Downregulation of FOXO1 in PCOS Granulosa Cells: A Direct Target of microRNA-183.
Article in Reproductive sciences (Thousand Oaks, Calif.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- The Cavβ4 subunit of Cav1.2 channels antagonizes isoproterenol-induced hypertrophy in rat cardiac muscle cells by down-regulating miR-183-5p.Physiological reports · 2026Article
- Androgen-Driven Dysregulation of Lipolysis in Cumulus Cells: Molecular Evidence and Metabolic Implications in Polycystic Ovary Syndrome.Reproductive medicine and biologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The purpose of this study is to investigate whether microRNA (miR-183) and forkhead box O1 (FOXO1) are abnormally expressed in polycystic ovary syndrome (PCOS) granulosa cells (GCs) and further explore its underlying molecular mechanisms. The expressions of miR-183 and FOXO1 in GCs isolated from 55 PCOS and 60 control patients were determined by quantitative reverse transcription-polymerase chain reaction (q-PCR) and western blot respectively. Granulosa-like tumor cell line (KGN) cells were used to alter the levels of miR-183 and FOXO1 by transfection. The target genes of miR-183 were first predicted by bioinformatics analysis and then verified by q-PCR, western blot and luciferase reporter assay. KGN cells were treated by insulin to further verify the relationship between miR-183 and FOXO1. The results demonstrated that the expression of miR-183 was significantly increased in PCOS patients, while FOXO1 was decreased. In addition, FOXO1 was a direct target of miR-183, which was negative with homeostasis model assessment for insulin resistance (HOMA-IR). Moreover, insulin treatment in KGN cells induced upregulation of miR-183 and decreased FOXO1. It could be speculated that insulin-mediated miR-183 targets FOXO1 to regulate the pathogenesis of PCOS, which might provide a new idea for investigating the functional role of miR-183 in PCOS GCs.
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Registered trials
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