Evidence map›Paper›PMID 40447865›Full record

ArticleInternational urogynecology journal2025

Regulation of Autophagy by the Cdk1/p53/p21 Feedback Loop in an Interstitial Cystitis/Bladder Pain Syndrome Cell Model: Implications for Inflammatory Response.

Kun Wang, Jian Shi, Zhen Chen, Yibo Zhuang, Dong Xue

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In one paragraph

Article in International urogynecology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kun WangDepartment of Surgical Urology, the Third Affiliated Hospital of Soochow University, Changzhou, 213003, Jiangsu, China.
Jian ShiDepartment of Surgical Urology, the Third Affiliated Hospital of Soochow University, Changzhou, 213003, Jiangsu, China.
Zhen ChenDepartment of Surgical Urology, the Third Affiliated Hospital of Soochow University, Changzhou, 213003, Jiangsu, China.
Yibo ZhuangDepartment of Pediatrics, the Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
Dong XueDepartment of Surgical Urology, the Third Affiliated Hospital of Soochow University, Changzhou, 213003, Jiangsu, China. 18921070933abc@sina.com.

Funding

Changzhou Municipal Science and Technology Bureau Basic Research project CJ20241112The Youth Talent Science and Technology Project of Changzhou Health Commission QN202307
6 · The paper itself

Abstract

introduction and hypothesisThe Cdk1/p53/p21 feedback loop could play an important role in maintaining a consistently high urinary tract epithelial permeability. This study is aimed at elucidating whether this feedback loop is associated with the inadequate autophagic response and the heightened inflammatory response in interstitial cystitis/bladder pain syndrome (IC/BPS).

methodsTo construct the IC/BPS cell model, HTB4 cells were first treated with 1% H

resultsCompared with normal urothelial HTB4 cells, the number of autophagosomes and the levels of LC3-I/II and Beclin 1 are significantly increased in IC/BPS cells. These cells also exhibit markedly higher levels of inflammatory markers (TNFα, IL-6, IL-8) and MDA, alongside notably reduced levels of the antioxidants GSH and SOD2. Compared with IC/BPS cells or IC/BPS cells co-transfected with p21 siRNA and Cdk1 control, the IC/BPS cells co-transfected with p21 siRNA and Cdk1 inhibitor exhibit a significant increase in terms of autophagosome numbers and the LC3-I/II level. Additionally, the levels of inflammatory factors are significantly decreased in the IC/BPS cells co-transfected with p21 siRNA and Cdk1 inhibitor.

conclusionIn IC/BPS, the Cdk1/p53/p21 feedback loop could regulate the level of autophagy and inflammatory response. This discovery identifies promising new therapeutic targets for IC/BPS, which may pave the way for innovative clinical approaches to this complex disorder.

Indexed as

AutophagyCDC2 Protein KinaseCyclin-Dependent Kinase Inhibitor p21Cystitis, InterstitialFeedback, PhysiologicalInflammationTumor Suppressor Protein p53Cell LineHumansCDC2 Protein KinaseCDK1 protein, humanCDKN1A protein, humanCyclin-Dependent Kinase Inhibitor p21TP53 protein, humanTumor Suppressor Protein p53AutophagyCdk1/p53/p21Feedback loopInflammatory responseInterstitial cystitis/bladder pain syndrome (IC/BPS)

Identifiers

PMID40447865

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.