Evidence map›Paper›PMID 40445993›Full record

ArticlePLoS neglected tropical diseases2025

Exposed nucleoprotein inside rabies virus particle as an ideal target for real-time quantitative evaluation of rabies virus particle integrity in vaccine quality control.

Jia Li, Yuhang Yang, Kuangrou Jia, Zhigao Zhang, Xiangmin Zhai, Yue Cao, Xinbiao Huang, Shouchun Cao, Yingsong Wu, Guanfeng Lin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS neglected tropical diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jia LiNational Institutes for Food and Drug Control, Beijing, China.
Yuhang YangInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
Kuangrou JiaInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
Zhigao ZhangInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
Xiangmin ZhaiInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
Yue CaoInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
Xinbiao HuangInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
Shouchun CaoNational Institutes for Food and Drug Control, Beijing, China.
Yingsong WuInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
Guanfeng LinInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.ORCID 0000-0002-1150-9625

Funding

Guangzhou Municipal Science and Technology Plan ProjectState Key Laboratory of Drug Regulatory Science Project
6 · The paper itself

Abstract

Currently, the integrity of rabies virus particle in quality control can only be assessed through electron microscopy. However, its time-consuming nature, operational complexity, limited accuracy and lack of quantification capability no longer meet the needs of modern vaccine production. Based on this, we developed a time-resolved fluoroimmunoassay (TRFIA) to enable real-time quantitative analysis of rabies virus particle integrity in human rabies vaccine, by detecting exposed nucleoprotein. Monoclonal antibodies against rabies glycoprotein and nucleoprotein were prepared using the classical hybridoma technology with the aim of constructing a novel detection approach for assessing particle integrity. A monoclonal antibody against rabies glycoprotein was immobilized on microplate wells to capture rabies virus particles, while a labeled antibody against the nucleoprotein served as the signal tracer. Multiple types of vaccine samples were analyzed to evaluate the effectiveness and accuracy of the developed TRFIA, combined with various virus particle destruction methods to validate its capability to assess particle integrity. The validation results were consistent with those obtained from electron microscopy. Therefore, this novel TRFIA offers a promising solution to address existing gaps in current analytical methods, enabling straightforward real-time, and quantitative evaluation of rabies virus particle integrity in the laboratory.

Indexed as

NucleoproteinsRabies VaccinesRabies virusVirionAnimalsAntibodies, MonoclonalAntibodies, ViralFluoroimmunoassayHumansQuality ControlRabiesAntibodies, MonoclonalAntibodies, ViralNucleoproteinsRabies Vaccines

Identifiers

PMID40445993
PMCPMC12124496

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.