Isolation and Characterization of Equine Mesenchymal Stromal/Stem Cells.

Lisa Marie Bosman, Melvin A Ambele, Michael S Pepper

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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2 · The registry

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Lisa Marie BosmanInstitute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

Melvin A AmbeleInstitute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

Michael S PepperInstitute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Regenerative medicine is a relatively new branch of therapeutics in equine medicine, which aims to restore and reconstitute tissue function and structure via cellular and/or noncellular approaches. Biological constituents such as mesenchymal stromal/stem cells (MSCs) are potent therapeutics, which can aid in damaged tissue regeneration due to their differentiation capacity into many different cell types such as adipose tissue, bone, and cartilage. MSCs can be successfully and conveniently isolated from equine subcutaneous adipose tissue (adipose-derived stromal cells, ASCs). In horses, there are currently no standardized methods for characterizing ASCs both in research and clinical practice. However, many studies are investigating antibodies which may be expressed on the ASC cell surface through multicolor immunophenotyping. This chapter describes techniques used to isolate, grow, characterize, and store equine ASCs, which have been adapted from extensive published research from the Institute of Cellular and Molecular Medicine (ICMM) at the University of Pretoria.

Indexed as

Cell SeparationMesenchymal Stem CellsAdipose TissueAnimalsCell Culture TechniquesCell DifferentiationCells, CulturedFlow CytometryHorsesImmunophenotypingAdipose tissueCell therapyEquineMesenchymal stromal cellsRegenerative medicine

Identifiers

PMID40445292

DOI10.1016/j.tvjl.2012.06.004

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.