ArticleMethods in molecular biology (Clifton, N.J.)2025
Differentiation of Adipose-Derived Stromal/Stem Cells into Osteocytes.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Adipose-Derived Stem Cells From Human Lumbar Subcutaneous Tissue in Obesity: Inflammatory Microenvironment Promotes Differentiation Capacity.Stem cells international · 2026Article
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Authors and funding
2 authors.
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Abstract
Osteogenesis, which refers to the formation of bone tissue, can be studied in vitro to elucidate the mechanisms of bone development, identify key factors and cytokines, and enhance potential regenerative medicine applications in tissue engineering and transplantation. Adipose-derived stromal/stem cells (ASCs) are particularly promising for these studies due to their capacity to differentiate into various mesodermal lineage cells, including osteoblasts. To induce osteogenic differentiation, ASCs are cultured in a complete growth medium (CGM) supplemented with β-glycerophosphate, ascorbate-2-phosphate and dexamethasone over a period of at least 3 weeks. Osteogenic differentiation can be assessed through the deposition of hydroxyapatite, a key component of mineralized bone matrix, and the activity of alkaline phosphatase, an enzyme crucial for matrix calcification. This study provides a protocol for differentiating ASCs into osteoblasts using osteogenic differentiation medium (ODM) and evaluating the differentiation using Alizarin Red S (ARS) staining and alkaline phosphatase (ALP) assays. The methodology includes the preparation of CGM and ODM, as well as the specific procedures for ARS staining and ALP activity assays. The ARS staining involves fixation of cells, staining, and quantification of hydroxyapatite deposition, while the ALP assay measures enzyme activity. Both assays are normalized to cell counts, determined through DAPI staining and microscopic analysis. The results of these assays are intended to provide an accurate evaluation of osteogenic differentiation efficiency in ASCs.
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