Evidence map›Paper›PMID 40445288›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Isolation of Adipose-Derived Mesenchymal Stromal/Stem Cells.

Anastasios Athanasiadis, Melvin A Ambele, Michael S Pepper

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Anastasios AthanasiadisInstitute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Melvin A AmbeleInstitute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Michael S PepperInstitute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa. michael.pepper@up.ac.za.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adipose-derived mesenchymal stromal/stem cells (AD-MSCs) offer a promising cell source for regenerative medicine due to their easy accessibility and potential application across a wide range of disorders. This chapter describes a standardized enzymatic isolation protocol to extract AD-MSCs from both solid adipose tissue and lipoaspirate. Adipose tissue samples are initially washed with phosphate-buffered saline (PBS) to remove blood contaminants. Tissue is then digested enzymatically with type I collagenase under controlled temperature and agitation to release the stromal vascular fraction (SVF) containing AD-MSCs. The enzyme activity is neutralized using complete growth medium followed by centrifugation to separate the cell pellet from unwanted material. The resulting cell pellet is resuspended and filtered to eliminate residual tissue fragments. After seeding the cells in culture, AD-MSCs in the SVF are characterized by adherence to plastic and the expression of specific cell surface marker (e.g., CD73, CD90, CD105), confirming their identity. This protocol provides a method for isolating viable AD-MSCs for downstream applications.

Indexed as

Adipose TissueCell SeparationMesenchymal Stem CellsBiomarkersCell Culture TechniquesCell DifferentiationCells, CulturedHumansBiomarkersAdipose tissueEnzymatic digestionIsolation protocolMesenchymal stromal/stem cellsPrimary cell isolationStromal vascular fraction

Identifiers

PMID40445288

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.