Evidence map›Paper›PMID 40445282›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Measurement of Intracellular Reactive Oxygen Species During Adipogenesis.

Ladislaus Tapiwa Adam, Melvin A Ambele, Michael S Pepper

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ladislaus Tapiwa AdamInstitute for Cellular and Molecular Medicine, Department of Immunology and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Melvin A AmbeleInstitute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa. melvin.ambele@up.ac.za.
Michael S PepperInstitute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reactive oxygen species (ROS) play an important role in the differentiation of immature stem cells to mature adipocytes in a process known as adipogenesis. Despite decades of study, there is still a gap in our understanding of the exact mechanisms played by ROS in regulating adipogenesis. This chapter describes flow cytometric measurement of endogenous ROS production during adipogenesis in adipose-derived stromal/stem cells (ASCs).

Indexed as

AdipogenesisFlow CytometryReactive Oxygen SpeciesAdipocytesAdipose TissueCell DifferentiationCells, CulturedHumansMesenchymal Stem CellsReactive Oxygen SpeciesAdipogenesisAdipose-derived stromal/stem cells (ASCsAdipose-derived stem/stromal cells (ASCs))CellROX® Deep Red reagentFlow cytometryMitoSOX™ RedReactive oxygen species

Identifiers

PMID40445282

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.