Evidence map›Paper›PMID 40445280›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Adipogenic Differentiation of Human Adipose-Derived Mesenchymal Stromal/Stem Cells (ASCs).

Ladislaus Tapiwa Adam, Reabetswe R Pitere, Melvin A Ambele, Michael S Pepper

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ladislaus Tapiwa AdamInstitute for Cellular and Molecular Medicine, Department of Immunology and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Reabetswe R PitereInstitute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Melvin A AmbeleInstitute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Michael S PepperInstitute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa. michael.pepper@up.ac.za.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adipose-derived stromal/stem cells (ASCs) from human fat tissue samples offer a valuable model to study adipogenesis in vitro. A hormonal cocktail that promotes growth and differentiation can be used in experimental procedures to facilitate the conversion of ASCs into adipocytes. This protocol describes methods for adipogenic differentiation of primary human ASCs in vitro, outlining flow cytometry quantification and fluorescence microscopy qualitative assessment of the adipogenic process.

Indexed as

AdipocytesAdipogenesisAdipose TissueCell DifferentiationMesenchymal Stem CellsCell Culture TechniquesCells, CulturedFlow CytometryHumansMicroscopy, Fluorescence3-Isobutyl-methylxanthineAdipogenesisAdipogenic differentiationAdipose-derived stromal/stem cellsFlow cytometryFluorescence microscopyIndomethacinRosiglitazone

Identifiers

PMID40445280

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.