ArticleMethods in molecular biology (Clifton, N.J.)2025
Adipogenic Differentiation from Human Embryonic Stem Cells Through Formation of Embryoid Bodies.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the context of adipogenesis, new adipocytes arise from the differentiation of pluripotent stem cells, including embryonic stem cells (hESCs). This process involves two fundamental phases: lineage commitment and terminal differentiation. This chapter elucidates a comprehensive 22-day protocol designed for the differentiation of the hESC cell line H9 toward the adipogenic lineage.During the initial phase, we initiate the differentiation process by forming embryoid bodies (EBs). This is achieved by culturing hESCs in an ultralow attachment plate for a duration of 12 days, during which the EBs are exposed to retinoic acid (RA) for a period of 3 days. Subsequently, adipocyte induction is facilitated by transferring EBs onto Geltrex-coated 6-well plates and subjecting them to adipogenic differentiation medium for an additional 10 days. To assess the successful internalization of lipids within the adipocytes, we employ Oil Red O staining. This protocol provides a robust framework for studying adipogenesis from pluripotent stem cells, offering insights into the intricate process of adipocyte development and serving as a valuable tool for related research endeavors.
Indexed as
Identifiers
40445274What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.