Evidence map›Paper›PMID 40445204›Full record

ArticleMicrobiology spectrum2025

Comparing Raman and NanoSIMS for heavy water labeling of single cells.

George A Schaible, John B Cliff, Jennifer A Crandall, Jeremy J Bougoure, Michael N Mathuri, Alex L Sessions, Joseph Atwood, Roland Hatzenpichler

Abstract readComparative Study
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

George A SchaibleDepartment of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, USA.ORCID 0000-0002-1031-4682
John B CliffEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.ORCID 0000-0002-7395-5604
Jennifer A CrandallCenter for Biofilm Engineering, Montana State University, Bozeman, Montana, USA.ORCID 0000-0002-9051-8481
Jeremy J BougoureEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.ORCID 0000-0002-4869-035X
Michael N MathuriDivision of Geological and Planetary Sciences, California Institute of Technology, Pasadena, California, USA.ORCID 0000-0003-0652-7789
Alex L SessionsDivision of Geological and Planetary Sciences, California Institute of Technology, Pasadena, California, USA.ORCID 0000-0001-6120-2763
Joseph AtwoodDepartment of Agricultural Economics and Economics, Montana State University, Bozeman, Montana, USA.
Roland HatzenpichlerDepartment of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, USA.ORCID 0000-0002-5489-3444

Funding

The need for speed: Stimulated Raman Spectroscopy for human gut microbiome researchR35GM147166 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Roland Hatzenpichler · 2022 to 2026
$1.9M
M J Murdock Charitable Trust SR-2017331NASA 80NSSC20K1365National Science Foundation 2125748National Science Foundation DBI-1726561NIGMS NIH HHS R35 GM147166NIH HHS 1R35GM147166-01
6 · The paper itself

Abstract

Stable isotope probing (SIP) experiments in conjunction with Raman microspectroscopy (Raman) or nano-scale secondary ion mass spectrometry (NanoSIMS) are frequently used to explore single cell metabolic activity in pure cultures as well as complex microbiomes. Despite the increasing popularity of these techniques, the comparability of isotope incorporation measurements using both Raman and NanoSIMS directly on the same cell remains largely unexplored. This knowledge gap creates uncertainty about the consistency of single-cell SIP data obtained independently from each method. Here, we conducted a comparative analysis of 543

Indexed as

Deuterium OxideEscherichia coliIsotope LabelingSingle-Cell AnalysisSpectrometry, Mass, Secondary IonSpectrum Analysis, RamanWaterDeuteriumDeuteriumDeuterium OxideWatercell activitycellular heterogeneityD2Oheavy watermetabolic activityNanoSIMSRamansingle cell microbiologystable isotope probingtechnique comparison

Identifiers

PMID40445204
PMCPMC12211084

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.