Evidence map›Paper›PMID 40444740›Full record

ArticleJournal of chromatographic science2025

Extension of an Ultra-High Performance Liquid Chromatography-MS/MS Method for the Determination of C3 Epimers of 25-Hydroxyl Derivatives of Vitamin D in Human Plasma.

Mohamed Abouzid, Julia Kerner, Aniceta Mikulska-Sauermann, Dorota Filipowicz, Matylda Resztak, Franciszek Główka, Leonid Kagan, Marta Karaźniewicz-Łada

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of chromatographic science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mohamed AbouzidDepartment of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 3 Rokietnicka Street, Poznań 60-806, Poland.
Julia KernerDepartment of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 3 Rokietnicka Street, Poznań 60-806, Poland.
Aniceta Mikulska-SauermannDepartment of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 3 Rokietnicka Street, Poznań 60-806, Poland.
Dorota FilipowiczDepartment of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, 49 Przybyszewskiego Street, Poznan 60-355, Poland.
Matylda ResztakDepartment of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 3 Rokietnicka Street, Poznań 60-806, Poland.
Franciszek GłówkaDepartment of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 3 Rokietnicka Street, Poznań 60-806, Poland.
Leonid KaganDepartment of Pharmaceutics and Center of Excellence for Pharmaceutical Translational Research and Education, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 0885, USA.
Marta Karaźniewicz-ŁadaDepartment of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 3 Rokietnicka Street, Poznań 60-806, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Current vitamin D quantification methods do not account for 25-hydroxyl epimers, which can falsely increase concentrations and mask actual deficiencies. Previously, we developed an ultra-high performance liquid chromatography-tandem mass spectrometry method to measure 25(OH)D3, 3-epi-25(OH)D3 and 25(OH)D2; here, we extended this method to include 3-epi-25(OH)D2. Analytes were separated using a Shimadzu UPLC with a Kinetex F5 column (100 × 2.1 mm, 2.6 μm). The mobile phase contained 0.1% formic acid in methanol and water (70:30, v/v). The internal standard, deuterated 25(OH)D3 and analytes were extracted with hexane. Detection was performed by a mass spectrometer equipped with a triple quadrupole after prior electrospray ionization. It demonstrated sufficient precision and spike recovery within and between days, with a coefficient of variation ≤15% and an error of determination ≤18%. The method exhibited linearity in the 2-100-ng/mL concentration range. The limits of quantification and limits of detection were 2 and 1 ng/mL, respectively. Extraction recoveries ranged from 70.05% to 97.13%. The matrix effect, carryover and dilution integrity were evaluated and met the FDA acceptance criteria. The stability of all metabolites in plasma was confirmed after 3 h of storage at room temperature and after three cycles of freezing at -80°C and thawing. Applying the method to clinical samples showed a high 25-hydroxyl epimer derived from vitamin D.

Indexed as

Tandem Mass SpectrometryVitamin DChromatography, High Pressure LiquidHumansLimit of DetectionLinear ModelsReproducibility of ResultsVitamin D

Identifiers

PMID40444740
PMCPMC12123413

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.