Evidence map›Paper›PMID 40444400›Full record

GuidelineInternational forum of allergy & rhinology2025

Metagenomics or Metataxonomics: Best Practice Methods to Uncover the Sinus Microbiome.

Isabella Burdon, George Bouras, Kevin Fenix, Kenny Yeo, James Connell, Clare Cooksley, Emma Barry, Sarah Vreugde, Peter J Wormald, Alkis J Psaltis

Abstract readGuideline
PubMed Full text at PMC Publisher
In one paragraph

Guideline in International forum of allergy & rhinology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it.

  1. Guideline
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Isabella BurdonDepartment of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, South Australia, Australia.ORCID 0000-0002-2464-7947
George BourasDepartment of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, South Australia, Australia.
Kevin FenixDepartment of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, South Australia, Australia.
Kenny YeoDepartment of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, South Australia, Australia.ORCID 0000-0001-5733-0463
James ConnellDepartment of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, South Australia, Australia.ORCID 0000-0002-3081-5433
Clare CooksleyDepartment of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, South Australia, Australia.
Emma BarryDepartment of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, South Australia, Australia.
Sarah VreugdeDepartment of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, South Australia, Australia.
Peter J WormaldDepartment of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, South Australia, Australia.
Alkis J PsaltisDepartment of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, South Australia, Australia.

Funding

Garnett Passe and Rodney Williams Memorial FoundationNational Health and Medical Research Council APP1196832
6 · The paper itself

Abstract

key pointsLong-read-metagenomic sequencing is the best method for analyzing the sinus microbiome. 16s-rRNA-sequencing (both long and short read) results in PCR amplification bias that significantly distorts the sinus microbiome.

Indexed as

MetagenomicsMicrobiotaParanasal SinusesSinusitisHumansRNA, Ribosomal, 16SRNA, Ribosomal, 16Sbacteriologychronic rhinosinusitissinusitis

Identifiers

PMID40444400
PMCPMC12401074

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.