Evidence map›Paper›PMID 40442873›Full record

ArticleNeuropathology and applied neurobiology2025

Multiplex Immunofluorescent Analysis of Alpha-Synuclein in Nigral Lewy Bodies With Heat-Induced Antibody Stripping Reveals an Intricate Multilayered Structure.

Dominik Hrabos, Satomi Hasegawa, Dorota Konickova

Abstract read
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In one paragraph

Article in Neuropathology and applied neurobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Dominik HrabosDepartment of Clinical and Molecular Pathology, Palacky University Olomouc and University Hospital Olomouc, Olomouc, Czech Republic.ORCID 0000-0001-9856-0126
Satomi HasegawaDepartment of Clinical and Molecular Pathology, Palacky University Olomouc and University Hospital Olomouc, Olomouc, Czech Republic.
Dorota KonickovaDepartment of Neurology, Palacky University Olomouc and University Hospital Olomouc, Olomouc, Czech Republic.ORCID 0000-0003-4446-0060

Funding

Ministry of Education, Youth and Science, Czech Republic LM2023050Ministry of Health, Czech Republic FNOl, 00098892
6 · The paper itself

Abstract

In the study, we employ an affordable, tissue-saving, and precise simultaneous multiplex immunofluorescence method with heat-induced antibody stripping to identify and structurally analyse nigral Lewy bodies in dopaminergic neurones. Analysis of different alpha-synuclein epitopes and proteoforms reveals an almost uniform, onion-like morphology of the Lewy bodies. The N-terminal and C-terminal domains are predominantly accessible to antibody binding in the peripheral shell of the bodies.

Indexed as

alpha-SynucleinLewy BodiesSubstantia NigraDopaminergic NeuronsFluorescent Antibody TechniqueHot TemperatureHumansLewy Body Diseasealpha-Synucleinalpha‐synucleinimmunofluorescenceLewy bodyLewy pathologyParkinson's disease

Identifiers

PMID40442873
PMCPMC12122903

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.