ArticleCommunications biology2025
Effect of pH on the cyanobacterial circadian oscillator in vitro.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Cyanobacterial Circadian Clock: Molecular Mechanisms and Physiological Outputs.Plants (Basel, Switzerland) · 2026Review
- Intrinsic period stability of the cyanobacterial circadian oscillator across in vitro and in vivo conditions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Temperature compensation of the Arabidopsis circadian clock.Npj biological timing and sleep · 2025Review
- Effect of pH on the cyanobacterial circadian oscillator in vitro.Communications biology · 2025Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The cyanobacterial clock protein KaiC exhibits robust 24-h oscillation of phosphorylation when incubated with KaiA, KaiB, and ATP in vitro. This study shows that the period of the in vitro phosphorylation rhythm of KaiC was correlated with solution pH, varying from 15 h at pH 6.5-36 h at pH 8.5, without affecting the period's temperature compensation. The solution pH altered the autophosphorylation and autodephosphorylation of KaiC and the effect of KaiB on KaiC but had little effect on its ATPase activity. It also modified the surface charge of the interface between two ATPase domains in KaiC, thereby affecting the autophosphorylation and autodephosphorylation activity of this protein via interdomain communication. These findings not only reveal key biochemical properties of the Kai oscillator but also provide insight into its evolutionary adaptation to environmental changes in cyanobacteria.
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Registered trials
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