ArticleScientific reports2025
Identification and characterization of a dsRNA-degrading nuclease CmdsRNase2 influencing RNAi efficiency in the rice leaffolder Cnaphalocrocis medinalis.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The rice leaffolder Cnaphalocrocis medinalis is one of the most important pests of rice. Double-stranded RNA-degrading enzyme (dsRNase) is one of key factors affecting the stability of dsRNA in insects, thus restricting the application of RNA interference (RNAi) technology in pest control. In this study, a dsRNase gene from C. medinalis, designated CmdsRNase2, was cloned by using reverse transcription-polymerase chain reaction (RT-PCR). The open reading frame (ORF) of CmdsRNase2 is 1,335 bp in length, encoding 444 amino acids. The CmdsRNase2 protein contains a signal peptide and an Endounuclease_NS domain that includes six active sites, one Mg
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