Evidence map›Paper›PMID 40441874›Full record

ArticleRNA (New York, N.Y.)2025

Human CCR4 deadenylase homolog Angel1 is a non-stop mRNA decay factor.

Tim Nicholson-Shaw, Megan E Dowdle, Yasmeen Ajaj, Mark Perelis, Amit Fulzele, Gene W Yeo, Eric J Bennett, Jens Lykke-Andersen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Tim Nicholson-ShawDivision of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA.ORCID 0000-0002-2484-5724
Megan E DowdleDivision of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA.
Yasmeen AjajDivision of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA.
Mark PerelisDepartment of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California 92093, USA.
Amit FulzeleDivision of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA.
Gene W YeoDepartment of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California 92093, USA.
Eric J BennettDivision of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA.
Jens Lykke-AndersenDivision of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA jlykkeandersen@ucsd.edu.

Funding

Functional RNA elements in the human genomeR01HG004659 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Kevin Daniel Corbett, Eugene Wei-Ming Yeo · 2008 to 2026
$12.4M
A Comprehensive Functional Map of Human Protein-RNA InteractionsU41HG009889 · NHGRI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI GRAVELEY, BRENTON R., YEO, EUGENE WEI-MING · 2018 to 2021
$9.4M
A comprehensive binding and functional map of human RNA-binding proteinsU24HG009889 · NHGRI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI GRAVELEY, BRENTON R., YEO, EUGENE WEI-MING · 2022 to 2025
$5.5M
Mechanisms of human RNA turnover and quality controlR35GM118069 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LYKKE-ANDERSEN, JENS · 2016 to 2025
$5.1M
Leveraging ubiquitin-dependent regulatory mechanisms to improve proteome quality in health and diseaseR35GM148339 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Eric J Bennett · 2023 to 2026
$2.1M
Defining the biological roles of the 2',3'-cyclic phosphatases Angel1 and Angel2F32GM151845 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Megan Elizabeth Dowdle · 2023 to 2026
$233k
Coupling of muscle blind-dependent RNA processing to the circadian clock in neuronal homeostasis and sleepF32HL143978 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PERELIS, MARK · 2019 to 2021
$146k
NHGRI NIH HHS R01 HG004659NHGRI NIH HHS U24 HG009889NHGRI NIH HHS U41 HG009889NHLBI NIH HHS F32 HL143978NIGMS NIH HHS F32 GM151845NIGMS NIH HHS R35 GM118069NIGMS NIH HHS R35 GM148339
6 · The paper itself

Abstract

Translation elongation stalls trigger mRNA decay and degradation of the nascent polypeptide via translation-dependent quality control pathways. One such pathway, non-stop mRNA decay (NSD), targets aberrant mRNAs that lack stop codons, for example, due to premature polyadenylation. Here we identify Angel1, a CCR4 deadenylase homolog whose biochemical activity remains poorly defined, as a rate-limiting factor for NSD in human cells. Angel1 associates with mRNA coding regions and proteins involved in ribosome-associated quality control and mRNA decay, consistent with a factor that monitors translation elongation stalls. Depletion of Angel1 causes stabilization of reporter mRNAs that are targeted for NSD by the absence of stop codons, but not an mRNA targeted for nonsense-mediated decay. A conserved catalytic residue of Angel1 is critical for its function in NSD. Our findings identify Angel1 as a human NSD factor and suggest that Angel1 catalytic activity plays a critical role in the NSD pathway.

Indexed as

ExoribonucleasesRibonucleasesRNA, MessengerRNA StabilityCodon, TerminatorHEK293 CellsHeLa CellsHumansNonsense Mediated mRNA DecayReceptors, CCR4CCR4 protein, humanCodon, TerminatorExoribonucleasesReceptors, CCR4RibonucleasesRNA, MessengerAngel1mRNA turnoverno-go decaynon-stop decayribosome quality control

Identifiers

PMID40441874
PMCPMC12265940

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.