Evidence map›Paper›PMID 40440896›Full record

ArticleJournal of hazardous materials2025

Environmental protein corona on nanoplastics altered the responses of skin keratinocytes and fibroblast cells to the particles.

Kayla Simpson, Leisha Martin, Shamus L O'Leary, John Watt, Seunghyun Moon, Tengfei Luo, Wei Xu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of hazardous materials, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kayla SimpsonDepartment of Life Sciences, College of Science, Texas A&M University - Corpus Christi, Corpus Christi, TX, USA.
Leisha MartinDepartment of Life Sciences, College of Science, Texas A&M University - Corpus Christi, Corpus Christi, TX, USA.
Shamus L O'LearyCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, NM, USA.
John WattCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, NM, USA.
Seunghyun MoonDepartment of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.
Tengfei LuoDepartment of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN 46556, USA; Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA; Center for Sustainable Energy of Notre Dame (ND Energy), University of Notre Dame, Notre Dame, IN 46556, USA.
Wei XuDepartment of Life Sciences, College of Science, Texas A&M University - Corpus Christi, Corpus Christi, TX, USA; Department of Veterinary Physiology & Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, USA. Electronic address: wxu1@tamu.edu.

Funding

Environmental effects on dermatoxicities of polycyclic aromatic hydrocarbonsR15ES030955 · NIEHS · TEXAS A&M UNIVERSITY-CORPUS CHRISTI · PI XU, WEI · 2019 to 2019
$401k
NIEHS NIH HHS R15 ES030955
6 · The paper itself

Abstract

The surface of nanoplastic particles (NPs) in the aquatic environment is always modified by organic matter in water, including protein components. The modifications are likely to affect interactions at the nano-bio interface, leading to an altered cellular response in humans. This study aimed to identify the changes in skin cell responses to the NPs conditioned by the proteins in natural seawater. The NPs were first conditioned by natural seawater, allowing an environmental corona to develop on the surface of the NPs. This was confirmed by electron microscopy. The cell internalization pathways of NPs with or without environmental corona were identified on skin keratinocytes and fibroblast cells by selective blockage of one of the four classic cell endocytic pathways. The intracellular locations of those NPs in keratinocytes and fibroblasts were demonstrated by co-localization of the particles and cell organelles. We also determined the genetic response of the keratinocytes to the environmentally conditioned NPs. The results showed that new internalization pathways by keratinocytes and fibroblast cells were activated by the environmental corona on NPs compared to the original NPs without surface corona. The surface corona of NPs also altered the NPs' intracellular locations in both keratinocytes and fibroblasts. The internalization of NPs with environmental corona induced the production of toll-like receptor 4, tumor necrosis factor alpha, cyclooxygenase-2, and interleukin 8 in keratinocytes. Since these factors are all involved in inflammatory responses and immune cell regulation, they can potentially trigger proinflammatory and chemotactic effects in human skin.

Indexed as

FibroblastsKeratinocytesMicroplasticsNanoparticlesProtein CoronaCell LineCyclooxygenase 2EndocytosisHumansInterleukin-8SeawaterSkinToll-Like Receptor 4Tumor Necrosis Factor-alphaCyclooxygenase 2Interleukin-8MicroplasticsProtein CoronaToll-Like Receptor 4Tumor Necrosis Factor-alphaDermal fibroblastEnvironmental coronaInflammationInternalizationIntracellular localizationKeratinocytesNanoplastics

Identifiers

PMID40440896
PMCPMC13528688

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.