Evidence map›Paper›PMID 40440420›Full record

ArticlePloS one2025

Identification and characterization of a target antigen recognized by the monoclonal antibody against Opisthorchis viverrini.

Anchalee Techasen, Chanika Worasith, Duangkamon Muengsaen, Jiraprapa Ponglong, Panupong Mahalapbutr, Napat Kongtaworn, Thanyada Rungrotmongkol, Kanoknan Khongsukwiwat, Phattharaphon Wongphutorn, Chompunoot Wangboon and 7 more

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In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Anchalee TechasenCholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.ORCID 0000-0002-4230-5641
Chanika WorasithCholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Duangkamon MuengsaenCholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Jiraprapa PonglongCholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Panupong MahalapbutrDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Napat KongtawornProgram in Bioinformatics and Computational Biology, Graduate School, Chulalongkorn University, Bangkok, Thailand.
Thanyada RungrotmongkolProgram in Bioinformatics and Computational Biology, Graduate School, Chulalongkorn University, Bangkok, Thailand.
Kanoknan KhongsukwiwatCholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Phattharaphon WongphutornCholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Chompunoot WangboonSchool of Preclinic, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
Chutima HomwonkCholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Sujittra ChaiyadetDepartment of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Thewarach LahaDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Sutas SuttiprapaDepartment of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Chadamas SakonsinsiriCholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.ORCID 0000-0003-0145-8753
Paiboon SithithawornCholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Raynoo ThananCholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Opisthorchis viverrini (Ov) infection caused opisthorchiasis, which posed an important risk for the development of cholangiocarcinoma (CCA). Therefore, it is crucial to focus on the primary prevention and control of opisthorchiasis in order to control CCA effectively in Thailand and other endemic regions. A recent diagnostic method of antigen detection using monoclonal antibody-based enzyme-linked immunosorbent assay (mAb-ELISA) has the potential for rapid mass screening of opisthorchiasis. Nevertheless, the specific antigen(s) in Ov adult worms recognized by mAb have not been determined. In this study, we aimed to identify and characterize the target molecule of our in-house Ov-specific monoclonal antibody (mAb KKU505). The specific antigenic band formed by the reaction of Ov adult worm extract and mAb KKU505 was detected using western blot analysis. The protein band was identified as the myosin heavy chain of Ov using LC-MS/MS analysis. The reactivity of the recombinant full-length myosin heavy chain (rMHC) was comparable to that of the crude Ov antigen when evaluated using mAb-ELISA at similar protein concentrations. Moreover, the binding ability between Ov myosin head domain and mAb KKU505 was confirmed using in silico analysis. The results reported here indicate that rMHC could potentially substitute for Ov crude antigen in antigen detection by mAb-ELISA and as a positive control for Ov-strip in lateral flow assays, thereby avoiding the use of laboratory animals for the production of Ov adult worms.

Indexed as

Antibodies, HelminthAntibodies, MonoclonalAntigens, HelminthOpisthorchiasisOpisthorchisAnimalsEnzyme-Linked Immunosorbent AssayHumansMiceMyosin Heavy ChainsAntibodies, HelminthAntibodies, MonoclonalAntigens, HelminthMyosin Heavy Chains

Identifiers

PMID40440420
PMCPMC12121735

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.