Evidence map›Paper›PMID 40439785›Full record

ArticleArchives of microbiology2025

Genomic and transcriptomic insights into the cellulose-degrading mechanism of Bacillus subtilis DC-11 and its novel cellulose catabolic pathway.

Chen Chen, Minqi Zhang, Yuanhao Zhang, Xueping Jiang, Jia Kong, Jieling Zhou, Gaiqun Huang, Ran Zhang, Hao Li, Zhongzheng Gui

Abstract read
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In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. A multi-enzyme producingFrontiers in microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Chen ChenJiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
Minqi ZhangJiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
Yuanhao ZhangJiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
Xueping JiangJiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
Jia KongJiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
Jieling ZhouJiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
Gaiqun HuangSericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong, 637000, Sichuan, China.
Ran ZhangJiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
Hao Li *Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China. lhoscarlh@just.edu.cn.
Zhongzheng Gui *Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China. srizzgui@hotmail.com.

Funding

the Graduate Research and Innovation Projects of Jiangsu Province No.KYCX24_4145the Jiangsu Agricultural Science and Technology Innovation Fund CX(24) 3084the National natural Science Foundatiion of China No.32302687
6 · The paper itself

Abstract

The accumulation of silkworm excrement poses a significant environmental challenge, contributing to pollution and resource squandering. Unraveling the novel mechanism governing bacterial cellulose degradation represents a vital avenue for augmenting cellulose conversion efficiency. This study investigated the cultivation of Bacillus subtilis DC-11 with different carbon sources, utilizing transcriptome sequencing to identify metabolic pathways and differentially expressed genes (DEGs) closely related to cellulose degradation. Transcriptome analysis revealed 3,917 DEGs between the carboxymethyl cellulose sodium (CMC-Na) treatment group and the glucose-supplemented (GLU) control group. Compared to the control group, the CMC-Na treatment group exhibited upregulation of 942 genes, while 1,996 genes were downregulated. KEGG pathway analysis of DEGs indicated the involvement of that carbohydrate metabolism and phosphotransferase system (PTS) pathways in response to cellulose degradation. Real-time quantitative PCR validation confirmed that the expressions of key genes, namely ytoP, bglH, gmuD, licH, licC, ywbA, licA, gmuA, and gmuB, associated with the PTS pathway were consistent with the transcriptomics data. These results suggest that B. subtilis DC-11 degrades cellulose via carbohydrate metabolism and PTS pathways. This study offers new insights into the cellulose metabolism pathway of B. subtilis DC-11, providing both a theoretical basis and innovative strategies for the efficient degradation of cellulose.

Indexed as

Bacillus subtilisCelluloseTranscriptomeBacterial ProteinsCarbohydrate MetabolismCarboxymethylcellulose SodiumGene Expression ProfilingGene Expression Regulation, BacterialGenomicsMetabolic Networks and PathwaysBacterial ProteinsCarboxymethylcellulose SodiumCelluloseBacillus subtilisCatabolic pathwayGenomicsSilkworm excrementTranscriptome

Identifiers

PMID40439785

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.