Evidence map›Paper›PMID 40439033›Full record

ArticleEnvironmental science & technology2025

Cryo-EM Structures of Apo and DDT-Bound P-Glycoprotein in Yellowfin Tuna.

Megan A Young, Steven D Rees, Sascha C T Nicklisch, Michael H B Stowell, Amro Hamdoun, Geoffrey Chang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Environmental science & technology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Megan A YoungSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0418, United States.ORCID 0000-0002-3330-3580
Steven D ReesSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0418, United States.ORCID 0000-0002-2258-7469
Sascha C T NicklischDepartment of Environmental Toxicology, University of California, Davis, One Shields Avenue, Davis, California 95616, United States.ORCID 0000-0003-3120-6485
Michael H B StowellMolecular, Cellular and Developmental Biology, University of Colorado Boulder, 1945 Colorado Avenue, Boulder, Colorado 80309, United States.
Amro HamdounCenter for Marine Biotechnology and Biomedical Science, Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0202, United States.
Geoffrey ChangSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0418, United States.ORCID 0000-0002-7476-9010

Funding

Identity, mechanisms and early life impacts of transporter interfering compoundsR01ES027921 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHANG, GEOFFREY A, HAMDOUN, AMRO M · 2018 to 2022
$3.0M
Molecular Biophysics Training Grant at UC San DiegoT32GM139795 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Galia Debelouchina, ELIZABETH A. KOMIVES · 2021 to 2026
$3.0M
Scripps Center for Oceans and Human Health: advancing the science of marine contaminants and seafood securityP01ES035541 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI AMRO M HAMDOUN, BRADLEY S MOORE · 2024 to 2026
$2.4M
Chameleon Sample Preparation Device for Cryo-EMS10OD032471 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LESCHZINER, ANDRES · 2022 to 2022
$420k
NIEHS NIH HHS P01 ES035541NIEHS NIH HHS R01 ES027921NIGMS NIH HHS T32 GM139795NIH HHS S10 OD032471
6 · The paper itself

Abstract

Persistent pollutants in the ocean impact the safety of seafood. Many emerging and legacy persistent organic pollutants (POPs) have been disposed into the world's oceans, exemplified by the recent discovery of large amounts of the halogenated pesticide dichlorodiphenyltrichloroethane (DDT) waste in the waters of Southern California. The biological mechanisms governing persistence and trophic transfer of marine pollutants into seafood species remain incompletely understood. Xenobiotic transporters, such as P-glycoprotein (P-gp), are present in all organisms and prevent the accumulation of toxic chemicals. Our previous work has demonstrated that halogenated marine pollutants can act as inhibitors of human and murine P-gp transporters by interacting with their binding site and impeding transport. Using cryo-EM, we determined the molecular interactions of DDT with P-glycoprotein from yellowfin tuna (

Indexed as

ATP Binding Cassette Transporter, Subfamily B, Member 1DDTTunaAnimalsBinding SitesCryoelectron MicroscopyWater Pollutants, ChemicalATP Binding Cassette Transporter, Subfamily B, Member 1DDTWater Pollutants, ChemicalABC transportersDDT pesticidemembrane transportersoceanic pollutionP-glycoproteinyellowfin tuna

Identifiers

PMID40439033
PMCPMC12164267

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.