ArticleFrontiers in veterinary science2025
Rapid detection of avipoxvirus using a fluorescent probe-based multienzyme isothermal amplification assay.
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- A rapid visual detection method for indel polymorphisms in the bovine PRNP gene based on a duplex MIRA-LFD assay.Frontiers in veterinary science · 2026Article
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9 authors.
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Abstract
Avipoxvirus (APV) is a prevalent DNA virus in avian species, causing clinical symptoms of fowlpox and leading to reduced egg production, slower broiler growth, and increased mortality. The spread of APV poses a significant threat to the global poultry industry, potentially causing substantial economic losses. Effective control of APV, particularly its major species such as fowlpoxvirus and pigeonpoxvirus, requires the development of rapid and specific diagnostic tools. In this study, a novel multi-enzyme isothermal rapid amplification (MIRA) assay was developed to detect APV. Various primer-probe combinations were screened to identify an optimal pair targeting a conserved region of the viral
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