ArticleFrontiers in veterinary science2025
Tricaine, eugenol and etomidate for repetitive procedural anesthesia in adult zebrafish,
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Zebrafish has emerged as a popular animal model in biomedical research. Numerous procedures and interventions require occasionally repetitive anesthesia. Tricaine is the most frequently used anesthetic for zebrafish and its efficacy is well established. However, the safety and efficacy of other anesthetics used for zebrafish require further examination, especially regarding repetitive anesthesia. Hence, we compare three anesthetics: tricaine (150 mg/l), eugenol (55 mg/l) and etomidate (4 mg/l) in wildtype adult zebrafish with and without interventions in the form of intraperitoneal injections. Groups of fish receiving the injections are named as (+ injection). We quantify anesthesia induction and recovery times as well as swimming behavior and cortisol levels as indicators of stress. Swimming behavior is quantified with the novel tank method as tank preference and number of turnings. Adult zebrafish are randomly divided into seven groups; tricaine (
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