ArticleFrontiers in microbiology2025
Microbial transformation of sewage sludge to biolipid-based fuel using potential oleaginous bacteria
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: The high proportion of sludge generation worldwide has sparked interest in utilizing it for alternative purposes. Among different potential applications, using sludge as a substrate for oleaginous bacteria is a relatively novel approach. The study was conducted to harness Methods: Sewage sludge was obtained from the I-9 treatment plant, Islamabad; after initial characterization the unprocessed sludge was optimized viz.: incubation time (24-96 h), inoculation rate (5-15%), pH levels (4-9), temperature (25-40°C), agitation (0-250 RPM), nitrogen sources (yeast, urea, ammonium chloride, and ammonium nitrate), and carbon sources (glucose, sucrose, starch, and dextrose). The qualitative analysis of the stored bio-lipids was performed using Fourier-transform infrared spectroscopy (FTIR) and Gas Chromatography Mass Spectrometry (GC-MS). Results and discussion: The maximum reactor performance was achieved with 40% lipid accumulation (gravimetric basis) in the dry cell biomass of
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