Evidence map›Paper›PMID 40438133›Full record

ArticleBio-protocol2025

Aneek Banerjee, Sandip Dey, Krishnamoorthi Srinivasan, Ankit Dhur, Priya Baid, Jayati Sengupta

Abstract read
In one paragraph

Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Aneek BanerjeeStructural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
Sandip DeyStructural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
Krishnamoorthi SrinivasanStructural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
Ankit DhurStructural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
Priya BaidStructural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
Jayati SenguptaStructural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ribosome, a complex macromolecular machine, plays a vital role in cellular translation. To investigate its structure and conduct in vitro experiments, isolating the ribosomes from cells is the first step. While isolating ribosomes from bacterial cells is routine, obtaining them from mycobacteria proves challenging due to the protective mycolic acid layer, which hinders cell lysis. In this study, we present a straightforward and efficient protocol for isolating ribosomes from

Indexed as

50S and 30S subunits70S ribosomeCo-sedimentationDensity gradient ultracentrifugationRibosomal subunit re-associationRibosome–protein interactiontRNA-free 70S ribosome

Identifiers

PMID40438133
PMCPMC12117347

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.