ArticleThe Journal of antimicrobial chemotherapy2025
Antibiotic stress affects the secretion and physicochemical features of extracellular vesicles produced by Helicobacter pylori.
Article in The Journal of antimicrobial chemotherapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed.
- Probiotic extracellular vesicles reprogram macrophage immunometabolism: From gut crosstalk to host health.Gut microbes · 2026Review
- Poultry handling-related stress alters membrane composition, extracellular vesicle production, survival, and virulence inApplied and environmental microbiology · 2026Article
- Berberine and Berberine-Derived Compounds as Promising Weapons AgainstPharmaceuticals (Basel, Switzerland) · 2026Review
- Synthesis and Characterization of Water-Soluble EDTA-Crosslinked Poly-β-Cyclodextrins Serving as Ion-Complexing Drug Carriers.Materials (Basel, Switzerland) · 2026Article
- Bacterial EVs contain small RNAs and transfer RNAs that regulate inflammation in lung infections.Frontiers in immunology · 2026Review
- Antibiotics Impact the Cytotoxicity and Cytopathic Effect ofInternational journal of molecular sciences · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
backgroundBacterial extracellular vesicles (EVs) may reduce the effectiveness of various antimicrobials; however, the impact of antibiotics on the secretion and properties of EVs produced by Helicobacter pylori has not been established.
methodsUsing clinical H. pylori strains and culture in EV-depleted media, the influence of ¼ × MIC of clarithromycin, metronidazole and levofloxacin on EV features was determined. Physicochemical properties of EVs were measured using nanoparticle tracking analysis and dynamic light scattering. Determination of fatty acid profiles of EVs and bacterial cells was performed with GC triple-quadrupole tandem MS. Bacteria and EVs were observed by scanning and transmission electron microscopy, respectively.
resultsAntibiotic stress induced in H. pylori affects the secretion intensity and physicochemical features of EVs secreted by this bacterium in a strain- and antibiotic-dependent manner. Exposure to ¼ × MIC of metronidazole or levofloxacin increased the secretion of EVs and contributed to significant changes in their fatty acid profile, whereas treatment with ¼ × MIC of clarithromycin did not induce such changes. Regardless of the culture conditions and the strain analysed, the existence of a conservative process of selective packaging of C17:0 fatty acids into EVs and a substantial limitation of this phenomenon for C14:0, C18:1 and C19c:0 was demonstrated.
conclusionThis is the first study showing the modulatory effect of antibiotic stress on the secretion and physicochemical features of EVs produced by H. pylori, as well as the first to suggest the involvement of EVs in maintaining the appropriate membrane fatty acid composition of this bacterium.
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