Evidence map›Paper›PMID 40437661›Full record

ArticleACS synthetic biology2025

Glycosylation of Structured Protein Domains in Cell-Free Reaction Environments.

Erik J Bidstrup, Kyle Hill, Chandra K Bandi, D Natasha Owitipana, Alina Chisti, Rochelle Aw, Xu Yang, Parastoo Azadi, Michael C Jewett, Lai-Xi Wang and 2 more

Abstract read
In one paragraph

Article in ACS synthetic biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Erik J BidstrupRobert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14853, United States.ORCID 0000-0001-9014-2042
Kyle HillCell-Free Protein Synthesis and Microbial Process Development, National Resilience Inc., Oakland, California 94606, United States.
Chandra K BandiRobert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14853, United States.
D Natasha OwitipanaDepartment of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 1220742, United States.ORCID 0000-0002-3701-3222
Alina ChistiRobert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14853, United States.
Rochelle AwDepartment of Bioengineering, Stanford University, Stanford, California 94305, United States.
Xu YangComplex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, Georgia 30602-4712, United States.ORCID 0000-0001-9802-9845
Parastoo AzadiComplex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, Georgia 30602-4712, United States.ORCID 0000-0002-6166-9432
Michael C JewettDepartment of Bioengineering, Stanford University, Stanford, California 94305, United States.ORCID 0000-0003-2948-6211
Lai-Xi WangDepartment of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 1220742, United States.ORCID 0000-0003-4293-5819
Weston KightlingerCell-Free Protein Synthesis and Microbial Process Development, National Resilience Inc., Oakland, California 94606, United States.
Matthew P DeLisaRobert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14853, United States.ORCID 0000-0003-3226-1566

Funding

National Glycoscience Resource- CCRC Service and Training - NGlycoR@CCRCR24GM137782 · NIGMS · UNIVERSITY OF GEORGIA · PI Parastoo Azadi · 2020 to 2026
$5.2M
Training future leaders at the chemistry-biology interfaceT32GM138826 · NIGMS · CORNELL UNIVERSITY · PI Jeremy Baskin · 2021 to 2026
$2.6M
NIGMS NIH HHS R24 GM137782NIGMS NIH HHS T32 GM138826
6 · The paper itself

Abstract

The production of

Indexed as

GlycoproteinsCell-Free SystemEscherichia coliGlycosylationHexosyltransferasesMembrane ProteinsProtein Domainsdolichyl-diphosphooligosaccharide - protein glycotransferaseGlycoproteinsHexosyltransferasesMembrane Proteinsasparagine-linked (N-linked) glycosylationcell-free protein synthesis (CFPS)glycoproteinimmunoglobulinprotein foldingsynthetic glycobiology

Identifiers

PMID40437661
PMCPMC12236189

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.