Evidence map›Paper›PMID 40437283›Full record

ArticleMikrochimica acta2025

Target-triggered hybrid chain amplified fluorescence aptasensor based on label-free dye and MnO

Hao Liang, Danliang Li, Shiyu Jia, Xuebing Zhang, Qinqin Bai, Ziyi Wang, Yujiao Cai, Jian Liu, Lili Chen

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. [Advances in Nanozyme-Aptamer Systems for the Detection of Foodborne Pathogens].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hao Liang *Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Danliang Li *Zhuzhou Hetang District Center for Disease Control and Prevention, Zhuzhou, Hunan, China.
Shiyu JiaDepartment of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Xuebing ZhangDepartment of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Qinqin BaiDepartment of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Ziyi WangDepartment of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Yujiao CaiDepartment of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Jian LiuDepartment of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Lili ChenDepartment of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China. chlili720612@163.com.

Funding

Natural Science Foundation of Hunan Province, China 2024JJ5344Scientific Research Foundation of Hunan Provincial Education Department, China 24B0412
6 · The paper itself

Abstract

Bacterial contamination poses significant threats to public health through food safety issues, creating a critical need for rapid and sensitive bacterial detection platforms. Herein, we developed a novel label-free fluorescent detection system leveraging target-triggered hybridization chain reaction (HCR) amplification, using Escherichia coli (E. coli) as a proof of concept. We engineered a hairpin-structured capture probe integrating an E. coli-specific aptamer with an HCR trigger sequence, achieving fluorescence amplification through three synergistic mechanisms: (1) HCR-driven DNA duplex assembly; (2) enhanced SYBR Green I (SG-I, a cost-effective non-labeled dye) intercalation into HCR-generated DNA duplexes; and (3) target-selective fluorescence modulation via MnO

Indexed as

Aptamers, NucleotideBiosensing TechniquesEscherichia coliFluorescent DyesManganese CompoundsNanostructuresOxidesBenzothiazolesDiaminesDNA, BacterialDNA, Single-StrandedLimit of DetectionMilkNucleic Acid HybridizationQuinolinesSpectrometry, FluorescenceAptamers, NucleotideBenzothiazolesDiaminesDNA, BacterialDNA, Single-StrandedFluorescent DyesManganese Compoundsmanganese dioxideOxidesQuinolinesSYBR Green IAptamerEscherichia coliFluorescenceHybridization chain reactionTarget-triggered amplification

Identifiers

PMID40437283

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.