ArticleAAPS PharmSciTech2025
Mitigating Shear Stress in Spray Drying for RNA-Loaded Lipid Nanoparticles through Process and Formulation Optimization.
Article in AAPS PharmSciTech, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Ambient-Stable mRNA Medicines: Emerging Paradigms in Dry and Solid-State Formulation.Pharmaceuticals (Basel, Switzerland) · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid nanoparticles (LNPs) have emerged as effective delivery systems for nucleic acid therapies, exemplified by the success of Moderna and Pfizer/BioNTech COVID-19 vaccines. However, these therapies still present significant shelf-life stability limitations, often requiring conservative cold chain storage conditions. Given the hurdles associated with cold chain supply, it is critical to overcome the stability challenges of these therapies. Drying technologies, such as spray drying (SD), can improve stability by removing water and preventing RNA hydrolysis and degradation. Nonetheless, shear and thermal stresses from SD can introduce additional risks to both the nucleic acid and the delivery system. Here, microfluidics was used to produce ribonucleic acid (RNA)-loaded LNPs with high encapsulation efficiency (> 95%), which were subsequently used to optimize the SD. A thorough process and formulation optimization was performed to maintain the LNPs' colloidal stability and nucleic acid encapsulation efficiency after spray drying. Poloxamer 188 (P188) proved crucial in protecting LNPs from shear stress possibly due to its ability to insert itself within lipid layers, allowing the maintenance of colloidal stability during SD. Additionally, SD was benchmarked against freeze drying (FD) as an alternative low shear drying technology. Overall, the study demonstrates the importance of optimizing the SD process to enhance nucleic acid-loaded LNPs' stability and delivery potential.
Indexed as
Identifiers
40437126What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.